On-chip multi-electrochemical sensor array platform for simultaneous screening of nitric oxide and peroxynitrite

In this work we report on the design, microfabrication and analytical performances of a new electrochemical sensor array (ESA) which allows for the first time the simultaneous amperometric detection of nitric oxide (NO) and peroxynitrite (ONOO(-)), two biologically relevant molecules. The on-chip de...

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Bibliographic Details
Published in:Lab on a chip 2011-04, Vol.11 (7), p.1342-1350
Main Authors: Quinton, Damien, Girard, Aurélie, Thi Kim, Loan To, Raimbault, Vincent, Griscom, Laurent, Razan, Florence, Griveau, Sophie, Bedioui, Fethi
Format: Article
Language:English
Subjects:
Chemistry Techniques, Analytical - instrumentation
Culture
Electrical measurements
Electrochemistry
Electrodes
Engineering Sciences
Equipment Design
Gold
Lab-On-A-Chip Devices
Micro and nanotechnologies
Microelectrodes
Microelectronics
Networks
Nitric oxide
Nitric Oxide - analysis
Nitric Oxide - chemistry
Peroxynitrous Acid - analysis
Peroxynitrous Acid - chemistry
Reactive fluid environment
Sensor arrays
Silver
Time Factors
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Summary:In this work we report on the design, microfabrication and analytical performances of a new electrochemical sensor array (ESA) which allows for the first time the simultaneous amperometric detection of nitric oxide (NO) and peroxynitrite (ONOO(-)), two biologically relevant molecules. The on-chip device includes individually addressable sets of gold ultramicroelectrodes (UMEs) of 50 µm diameter, Ag/AgCl reference electrode and gold counter electrode. The electrodes are separated into two groups; each has one reference electrode, one counter electrode and 110 UMEs specifically tailored to detect a specific analyte. The ESA is incorporated on a custom interface with a cell culture well and spring contact pins that can be easily interconnected to an external multichannel potentiostat. Each UME of the network dedicated to the detection of NO is electrochemically modified by electrodepositing thin layers of poly(eugenol) and poly(phenol). The detection of NO is performed amperometrically at 0.8 V vs. Ag/AgCl in phosphate buffer solution (PBS, pH = 7.4) and other buffers adapted to biological cell culture, using a NO-donor. The network of UMEs dedicated to the detection of ONOO(-) is used without further chemical modification of the surface and the uncoated gold electrodes operate at -0.1 V vs. Ag/AgCl to detect the reduction of ONOOH in PBS. The selectivity issue of both sensors against major biologically relevant interfering analytes is examined. Simultaneous detection of NO and ONOO(-) in PBS is also achieved.
ISSN:1473-0197
1473-0189
DOI:10.1039/c0lc00585a