Effect of Mitochondrial Dysfunction on Carbon Metabolism and Gene Expression in Flower Tissues of Arabidopsis thaliana

We characterized the transcriptomic response of transgenic plants carrying a mitochondrial dysfunction induced by the expression of the unedited form of the ATP synthase subunit 9. The u-ATP9 transgene driven by A9 and APETALA3 promoters induce mitochondrial dysfunction revealed by a decrease in bot...

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Bibliographic Details
Published in:Molecular plant 2011, Vol.4 (1), p.127-143
Main Authors: Busi, Maria V., Gomez-Lobato, Maria E., Rius, Sebastian P., Turowski, Valeria R., Casati, Paula, Zabaleta, Eduardo J., Gomez-Casati, Diego F., Araya, Alejandro
Format: Article
Language:chi ; eng
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
ATP合成酶
Carbon
Carbon - metabolism
Cellular biology
Electron transport
Flowers - genetics
Flowers - growth & development
Flowers - metabolism
Gene expression
Gene Expression Regulation, Plant
Life Sciences
Metabolism
Microbiology and Parasitology
Mitochondria - genetics
Mitochondria - metabolism
Proteins
Signal Transduction
transcriptome analysis
功能障碍
基因表达
拟南芥
氧化应激反应
碳代谢
线粒体呼吸
转录水平
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Summary:We characterized the transcriptomic response of transgenic plants carrying a mitochondrial dysfunction induced by the expression of the unedited form of the ATP synthase subunit 9. The u-ATP9 transgene driven by A9 and APETALA3 promoters induce mitochondrial dysfunction revealed by a decrease in both oxygen uptake and adenine nucleo- tides （ATP, ADP） levels without changes in the ATIP/ADP ratio. Furthermore, we measured an increase in ROS accumulation and a decrease in glutathione and ascorbate levels with a concomitant oxidative stress response. The transcriptome analysis of young Arabidopsis flowers, validated by qRT-PCR and enzymatic or functional tests, showed dramatic changes in u-ATP9 plants. Both lines display a modification in the expression of various genes involved in carbon, lipid, and cell wall metabolism, suggesting that an important metabolic readjustment occurs in plants with a mitochondrial dysfunction. Interestingly, transcript levels involved in mitochondrial respiration, protein synthesis, and degradation are affected. More- over, the levels of several mRNAs encoding for transcription factors and DNA binding proteins were also changed. Some of them are involved in stress and hormone responses, suggesting that several signaling pathways overlap. Indeed, the tran- scriptome data revealed that the mitochondrial dysfunction dramatically alters the expression of genes involved in signaling pathways, including those related to ethylene, absicic acid, and auxin signal transduction. Our data suggest that the mitochondrial dysfunction model used in this report may be useful to uncover the retrograde signaling mechanism between the nucleus and mitochondria in plant cells.
ISSN:1674-2052
1752-9867
DOI:10.1093/mp/ssq065