Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium

Efficient implementation of an environmental biomarker requires multi-annual comparability over a wide geographical range. The present study improved the comparability of a quantitative competitive metallothionein (MT) enzyme-linked-immuno-sorbent-assay (ELISA) in the sentinel fish Lithognathus morm...

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Bibliographic Details
Published in:Marine environmental research 2008-06, Vol.65 (5), p.427-436
Main Authors: Yudkovski, Yana, Rogowska-Wrzesinska, Adelina, Yankelevich, Irena, Shefer, Edna, Herut, Barak, Tom, Moshe
Format: Article
Language:English
Subjects:
Actins - genetics
Actins - metabolism
Agnatha. Pisces
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Beta-actin
Biological and medical sciences
Biomarker
Biomarkers
Biomonitoring
Cadmium
Cadmium - metabolism
Cadmium - toxicity
Ecosystem
ELISA
Environmental Monitoring
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Lithognathus mormyrus
Liver - chemistry
Marine
Mediterranean
Metallothionein
Metallothionein - analysis
Metallothionein - biosynthesis
Metallothionein - genetics
Metallothionein - immunology
Perciformes - genetics
Perciformes - metabolism
Real time PCR
Recombinant Proteins - immunology
Sea water ecosystems
Synecology
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
Water Pollutants - metabolism
Water Pollutants - toxicity
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Summary:Efficient implementation of an environmental biomarker requires multi-annual comparability over a wide geographical range. The present study improved the comparability of a quantitative competitive metallothionein (MT) enzyme-linked-immuno-sorbent-assay (ELISA) in the sentinel fish Lithognathus mormyrus by introducing to the assay recombinant MT and beta-actin standards. Commercial antibodies for cod MT and mammalian actin were implemented. In addition, a sensitive anti L. mormyrus MT antibody was produced, adequate only for solid phase immunochemical assays. Cadmium was applied to the fish through injection and feeding to serve as a testing platform of the ELISA. The results demonstrated high potential protective capacity of the liver against toxic levels of transition metals through increasing MT levels. MT transcript levels were evaluated also from fish sampled at polluted and relatively clean natural sites, indicating applicability of MT as biomarker of exposure to a multi-factorial pollution, in comparison to its low revealed sensitivity to controlled cadmium exposure.
ISSN:0141-1136
1879-0291
DOI:10.1016/j.marenvres.2008.02.001