Sequence of the Small Subunit Ribosomal RNA Gene of Perkinsus atlanticus-like Isolated from Carpet Shell Clam in Galicia, Spain

Parasites identified as Perkinsus atlanticus have been reported infecting carpet shell clams in Galicia (northwest Spain). We have sequenced the 18S ribosomal RNA gene of in vitro cultured Perkinsus atlanticus-like or hypnospores from diseased clams, and compared it with the same genomic region from...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2000-09, Vol.2 (5), p.419-428
Main Authors: Figueras, Antonio, Lorenzo, Gema, Ordás, M Camino, Gouy, Manolo, Novoa, Beatriz
Format: Article
Language:English
Subjects:
Anurofeca
Dermocystidium
Divergence
Fungi
Genomics
Hypnospores
Ichthyophonus
Life Sciences
Marine
Mesomycetozoa
Mollusks
Nucleic acids
Nucleotide sequence
Other
Parasites
Perkinsus atlanticus
Phylogeny
Protists
Psorospermium
Ribonucleic acid
RNA
rRNA 18S
rRNA 5.8S
Ruditapes decussatus
Sequencing
Spacer
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Summary:Parasites identified as Perkinsus atlanticus have been reported infecting carpet shell clams in Galicia (northwest Spain). We have sequenced the 18S ribosomal RNA gene of in vitro cultured Perkinsus atlanticus-like or hypnospores from diseased clams, and compared it with the same genomic region from P. marinus and Perkinsus sp. We have also compared the sequence of internal transcribed spacer (ITS) 1, ITS 2, and 5.8S rRNA from our isolate with the P. atlanticus GenBank sequence. The phylogenetic analysis of our cultured parasite based on the 18S gene led us to conclude that this isolate is not related to the genus Perkinsus but to the protists Anurofeca, Ichthyophonus, and Psorospermium, located near the animal-fungal divergence. These last two genera have been included, together with Dermocystidium, in the newly described DRIPs (Dermocystidium, rossete agent, Ichthyophonus, and Psorospermium) clade, recently named Mesomycetozoa.
ISSN:1436-2228
1436-2236
DOI:10.1007/s101260000015