Mucosal immunization against ovine lentivirus using PEI–DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes

Abstract Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)–DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral re...

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Bibliographic Details
Published in:Vaccine 2008-08, Vol.26 (35), p.4494-4505
Main Authors: Reina, R, Barbezange, C, Niesalla, H, de Andrés, X, Arnarson, H, Biescas, E, Mazzei, M, Fraisier, C, McNeilly, T.N, Liu, C, Perez, M, Carrozza, M.L, Bandecchi, P, Solano, C, Crespo, H, Glaria, I, Huard, C, Shaw, D.J, de Blas, I, de Andrés, D, Tolari, F, Rosati, S, Suzan-Monti, M, Andrésdottir, V, Torsteinsdottir, S, Petursson, G, Lujan, L, Pepin, M, Amorena, B, Blacklaws, B, Harkiss, G.D
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Viral
Antibodies, Viral - blood
Applied microbiology
Biological and medical sciences
Cell Proliferation
Cytotoxicity Tests, Immunologic
DNA vaccination
Female
Fundamental and applied biological sciences. Psychology
Gene Products, env
Gene Products, env - genetics
Gene Products, env - immunology
Gene Products, pol
Gene Products, pol - genetics
Gene Products, pol - immunology
Genetic Vectors
Immunology
Lentivirus
Leukocytes, Mononuclear
Leukocytes, Mononuclear - immunology
Life Sciences
Lung
Lung - immunology
Lung - pathology
Lung - virology
Male
Microbiology
Miscellaneous
Mucosal
Nasopharynx
Nasopharynx - immunology
Ovine lentivirus
Pneumonia, Progressive Interstitial, of Sheep
Pneumonia, Progressive Interstitial, of Sheep - prevention & control
Proviruses
Proviruses - isolation & purification
Severity of Illness Index
Sheep
T-Lymphocytes, Cytotoxic
T-Lymphocytes, Cytotoxic - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Vaccinia virus
Vaccinia virus - genetics
Vaccinology
Viral Load
Viral Vaccines
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Virology
Visna-maedi virus
Visna-maedi virus - immunology
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Summary:Abstract Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)–DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral responses prior to but not after VMV challenge. Systemic T cell proliferative and cytotoxic responses were generally low, with the responses following single gag gene immunization being significantly depressed after challenge. A transient reduction in provirus load in the blood early after challenge was observed following env immunization, whilst the gag gene either alone or in combination with env resulted in significantly elevated provirus loads in lung. However, despite this, a significant reduction in lesion score was observed in animals immunized with the single gag gene at post-mortem. Inclusion of IFN-γ in the immunization mixture in general had no significant effects. The results thus showed that protective effects against VMV-induced lesions can be induced following respiratory immunization with the single gag gene, though this was accompanied by an increased pulmonary provirus load.
ISSN:0264-410X
1873-2518
0264-410X
DOI:10.1016/j.vaccine.2008.06.065