Comparative proteomic analysis of human mesenchymal and embryonic stem cells: Towards the definition of a mesenchymal stem cell proteomic signature

Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue,...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2009-01, Vol.9 (2), p.223-232
Main Authors: Roche, Stephane, Delorme, Bruno, Oostendorp, Robert A.J, Barbet, Romain, Caton, David, Noel, Daniele, Boumediene, Karim, Papadaki, Helen A, Cousin, Beatrice, Crozet, Carole, Milhavet, Ollivier, Casteilla, Louis, Hatzfeld, Jacques, Jorgensen, Christian, Charbord, Pierre, Lehmann, Sylvain
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Analysis of Variance
Analytical, structural and metabolic biochemistry
Antigens, CD - metabolism
Biochemistry
Biochemistry, Molecular Biology
Biological and medical sciences
Bone marrow
Bone Marrow - chemistry
Cells, Cultured
Cellular Biology
Databases, Protein
Electrophoresis, Gel, Two-Dimensional
Embryonic stem cell
Embryonic Stem Cells - chemistry
Embryonic Stem Cells - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression
Genomics
Humans
Life Sciences
Mesenchymal stem cell
Mesenchymal Stromal Cells - chemistry
Mesenchymal Stromal Cells - metabolism
Miscellaneous
Phenotype
Proteins
Proteins - analysis
Proteins - isolation & purification
Proteomics - methods
Subcellular Processes
Synovial Membrane - cytology
Umbilical vein
Umbilical Veins - cytology
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Summary:Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2-DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo-natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200800035