Identification of candidate antigen in Whipple's disease using a serological proteomic approach

Whipple's disease (WD) is a chronic multisystemic infection, caused by Tropheryma whipplei, a Gram‐positive rod. Recently, a reliable method has been developed for cultivating T. whipplei in vitro. This together with the availability of complete genome sequence of T. whipplei prompted us to ini...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2006-06, Vol.6 (11), p.3294-3305
Main Authors: Kowalczewska, Malgorzata, Fenollar, Florence, Lafitte, Daniel, Raoult, Didier
Format: Article
Language:English
Subjects:
Actinomycetales - metabolism
Analytical, structural and metabolic biochemistry
Antigens - immunology
Biological and medical sciences
Biomarkers
Biotechnology
Cells, Cultured
Electrophoresis, Gel, Two-Dimensional
Endocarditis, Bacterial - metabolism
Endocarditis, Bacterial - microbiology
Fundamental and applied biological sciences. Psychology
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunoblotting
Life Sciences
Medical sciences
Miscellaneous
Other diseases. Semiology
Proteins
Proteome
Serological diagnosis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tropheryma whipplei
Two-dimensional electrophoresis
Whipple Disease - immunology
Whipple Disease - microbiology
Whipple's disease
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Summary:Whipple's disease (WD) is a chronic multisystemic infection, caused by Tropheryma whipplei, a Gram‐positive rod. Recently, a reliable method has been developed for cultivating T. whipplei in vitro. This together with the availability of complete genome sequence of T. whipplei prompted us to initiate proteome analysis of T. whipplei. The objective of the present study was to identify candidate proteins for serological diagnosis of WD. Immunoreactivities of sera collected from 18 patients with WD were compared with those of 24 control subjects who did not have WD. For this, we used 2‐DE, immunoblotting, and MS. In total, we identified 23 candidate antigenic proteins. These included a subset of six proteins, each of which was found significantly more frequently in cases as compared to their controls. The remaining 17 proteins were found exclusively in cases. The methods we used in the current study enabled us to identify candidate antigens that, in our view, might be useful for serological diagnosis of WD.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500171