Use of coherent control for selective two-photon fluorescence microscopy in live organisms

We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching be...

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Bibliographic Details
Published in:Optics express 2006-01, Vol.14 (2), p.759-766
Main Authors: Ogilvie, Jennifer P, Débarre, Delphine, Solinas, Xavier, Martin, Jean-Louis, Beaurepaire, Emmanuel, Joffre, Manuel
Format: Article
Language:English
Subjects:
Bioengineering
Life Sciences
Optics
Physics
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Summary:We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy.
ISSN:1094-4087
1094-4087
DOI:10.1364/opex.14.000759