Antithrombotic Effect of Oil from the Pulp of Bocaiúva—Acrocomia aculeata

The study aimed to evaluate the antithrombotic action of Acrocomia aculeata pulp oil (AAPO) in natura, in an in vitro experimental model. AAPO was obtained by solvent extraction, and its chemical characterization was performed by gas chromatography coupled to a mass spectrometer (GC-MS). In vitro to...

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Bibliographic Details
Published in:Nutrients 2024-07, Vol.16 (13)
Main Authors: Espinoça, Isabelly Teixeira, Basilio, Denise Caroline Luiz Soares, de Araujo, Anna Júlia Papa, Ota, Rafael Seiji Nakano, de Souza, Kamylla Fernanda Souza, Cassemiro, Nadla Soares, Lagatta, Davi Campos, Paredes-Gamero, Edgar Julian, Macedo, Maria Lígia Rodrigues, Silva, Denise Brentan, Sardi, Janaina de Cássia Orlandi, Wilhelm-Filho, Danilo, Jacobowski, Ana Cristina, Parisotto, Eduardo Benedetti
Format: Article
Language:English
Subjects:
Chemical properties
Composition
Health aspects
Palms
Testing
Thrombolytic drugs
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Summary:The study aimed to evaluate the antithrombotic action of Acrocomia aculeata pulp oil (AAPO) in natura, in an in vitro experimental model. AAPO was obtained by solvent extraction, and its chemical characterization was performed by gas chromatography coupled to a mass spectrometer (GC-MS). In vitro toxicity was evaluated with the Trypan Blue exclusion test and in vivo by the Galleria mellonella model. ADP/epinephrine-induced platelet aggregation after treatment with AAPO (50, 100, 200, 400, and 800 μg/mL) was evaluated by turbidimetry, and coagulation was determined by prothrombin activity time (PT) and activated partial thromboplastin time (aPTT). Platelet activation was measured by expression of P-selectin on the platelet surface by flow cytometry and intraplatelet content of reactive oxygen species (ROS) by fluorimetry. The results showed that AAPO has as major components such as oleic acid, palmitic acid, lauric acid, caprylic acid, and squalene. AAPO showed no toxicity in vitro or in vivo. Platelet aggregation decreased against agonists using treatment with different concentrations of AAPO. Oil did not interfere in PT and aPTT. Moreover, it expressively decreased ROS-induced platelet activation and P-selectin expression. Therefore, AAPO showed antiplatelet action since it decreased platelet activation verified by the decrease in P-selectin expression as well as in ROS production.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu16132024