In Vitro Antioxidant and In Silico Evaluation of the Anti-β-Lactamase Potential of the Extracts of ICylindrospermum alatosporum/I NR125682 and ILoriellopsis cavenicola/I NR117881

Cyanobacteria in recent times have been touted to be a suitable source for the discovery of novel compounds, including antioxidants and antibiotics, due to their large arsenal of metabolites. This study presents the in vitro antioxidant and in silico evaluation of Cylindrospermum alatosporum NR12568...

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Bibliographic Details
Published in:Antioxidants 2024-05, Vol.13 (5)
Main Authors: Ikhane, Albert O, Sithole, Siphesihle Z, Cele, Nkosinathi D, Osunsanmi, Foluso O, Mosa, Rebamang A, Opoku, Andrew R
Format: Article
Language:English
Subjects:
Antibiotics
Antioxidants
Drug resistance in microorganisms
Ethylenediaminetetraacetic acid
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Summary:Cyanobacteria in recent times have been touted to be a suitable source for the discovery of novel compounds, including antioxidants and antibiotics, due to their large arsenal of metabolites. This study presents the in vitro antioxidant and in silico evaluation of Cylindrospermum alatosporum NR125682 and Loriellopsis cavenicola NR117881, isolated from freshwater ponds around the campus of the University of Zululand, South Africa. The isolates were confirmed using 16S rRNA. Various crude extracts of the isolated microbes were prepared through sequential extraction using hexane, dichloromethane, and 70% ethanol. The chemical constituents of the crude extracts were elucidated by FTIR and GC-MS spectroscopy. The antioxidant potential of the extracts was determined by the free radical (DPPH, ABTS, [sup.•]OH, and Fe[sup.2+]) systems. Molecular docking of the major constituents of the extracts against β-lactamase was also evaluated. GC-MS analysis indicated the dominating presence of n-alkanes. The extracts exhibited varying degrees of antioxidant activity (scavenging of free radicals; an IC[sub.50] range of 8–10 µg/mL was obtained for ABTS). A good binding affinity (−6.6, −6.3 Kcal/mol) of some the organic chemicals (diglycerol tetranitrate, and 2,2-dimethyl-5-(3-methyl-2-oxiranyl)cyclohexanone) was obtained following molecular docking. The evaluated antioxidant activities, coupled with the obtained docking score, potentiates the antimicrobial activity of the extracts.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13050608