Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel Wound Dressing for Specific Removal and Efficient Eradication of IPseudomonas aeruginosa/I

Here we present for the first time a potential wound dressing material implementing aptamers as binding entities to remove pathogenic cells from newly contaminated surfaces of wound matrix-mimicking collagen gels. The model pathogen in this study was the Gram-negative opportunistic bacterium Pseudom...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-03, Vol.24 (5)
Main Authors: Kraemer, Markus, Bellion, Magali, Kissmann, Ann-Kathrin, Herberger, Tilmann, Synatschke, Christopher V, Bozdogan, Anil, Andersson, Jakob, Rodriguez, Armando, Ständker, Ludger, Wiese, Sebastien, Stenger, Steffen, Spellerberg, Barbara, Gottschalk, Kay-Eberhard, Cetinkaya, Ahmet, Pietrasik, Joanna, Weil, Tanja, Rosenau, Frank
Format: Article
Language:English
Subjects:
Drug delivery systems
Drugs
Health aspects
Infection
Peptides
Pseudomonas aeruginosa
Vehicles
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Summary:Here we present for the first time a potential wound dressing material implementing aptamers as binding entities to remove pathogenic cells from newly contaminated surfaces of wound matrix-mimicking collagen gels. The model pathogen in this study was the Gram-negative opportunistic bacterium Pseudomonas aeruginosa, which represents a considerable health threat in hospital environments as a cause of severe infections of burn or post-surgery wounds. A two-layered hydrogel composite material was constructed based on an established eight-membered focused anti-P. aeruginosa polyclonal aptamer library, which was chemically crosslinked to the material surface to form a trapping zone for efficient binding of the pathogen. A drug-loaded zone of the composite released the C14R antimicrobial peptide to deliver it directly to the bound pathogenic cells. We demonstrate that this material combining aptamer-mediated affinity and peptide-dependent pathogen eradication can quantitatively remove bacterial cells from the "wound" surface, and we show that the surface-trapped bacteria are completely killed. The drug delivery function of the composite thus represents an extra safeguarding property and thus probably one of the most important additional advances of a next-generation or smart wound dressing ensuring the complete removal and/or eradication of the pathogen of a freshly infected wound.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms24054800