Verification of the Interaction Target Protein of the Effector ApCE22 of IArthrinium phaeospermum/I in IBambusa pervariabilis × Dendrocalamopsis grandis/I

The study of interaction proteins of the pathogen A. phaeospermum effector protein is an important means to analyze the disease-resistance mechanism of Bambusa pervariabilis × Dendrocalamopsis grandis shoot blight. To obtain the proteins interacting with the effector ApCE22 of A. phaeospermum, 27 pr...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2023-03, Vol.13 (4)
Main Authors: Fang, Xinmei, Yan, Peng, Owusu, Adjei Mark, Zhu, Tianhui, Li, Shujiang
Format: Article
Language:English
Subjects:
Analysis
Ascomycota
Bacterial proteins
Bamboo
Diseases and pests
Fungi
Physiological aspects
Plant immunology
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Summary:The study of interaction proteins of the pathogen A. phaeospermum effector protein is an important means to analyze the disease-resistance mechanism of Bambusa pervariabilis × Dendrocalamopsis grandis shoot blight. To obtain the proteins interacting with the effector ApCE22 of A. phaeospermum, 27 proteins interacting with the effector ApCE22 were initially identified via a yeast two-hybrid assay, of which four interaction proteins were obtained after one-to-one validation. The B2 protein and the chaperone protein DnaJ chloroplast protein were then verified to interact with the ApCE22 effector protein by bimolecular fluorescence complementation and GST pull-down methods. Advanced structure prediction showed that the B2 protein contained the DCD functional domain related to plant development and cell death, and the DnaJ protein contained the DnaJ domain related to stress resistance. The results showed that both the B2 protein and DnaJ protein in B. pervariabilis × D. grandis were the target interaction proteins of the ApCE22 effector of A. phaeospermum and related to the stress resistance of the host B. pervariabilis × D. grandis. The successful identification of the pathogen effector interaction target protein in B. pervariabilis × D. grandis plays an important role in the mechanism of pathogen–host interaction, thus providing a theoretical basis for the control of B. pervariabilis × D. grandis shoot blight.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom13040590