Correlation of Oxidative Stress with Global DNA Methylation in Type 2 DM

The incidence and prevalence of Type 2 Diabetes Mellitus (T2DM) is increasing. T2DM is a multifactorial, polygenetic disease due to impaired insulin secretion and insulin resistance. Single nucleotide polymorphisms explain less than 20% of disease progression and heritability. Various studies have a...

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Published in:Indian journal of clinical biochemistry 2022-05, Vol.34 (S1), p.S198
Main Authors: Satpathy, Amrita, Rattan, Roma, Sahu, Nirmal Chandra, Mandal, M.K, Das, Upendra Kumar, Sarangi, Pratikhya, Pradhan, Devi Prasad
Format: Article
Language:English
Subjects:
Chromosomes
Development and progression
Diabetes therapy
DNA
Enzyme-linked immunosorbent assay
Epigenetic inheritance
Genetic polymorphisms
Genetic research
Insulin resistance
Medical research
Medicine, Experimental
Methylation
Oxidative stress
Type 2 diabetes
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Summary:The incidence and prevalence of Type 2 Diabetes Mellitus (T2DM) is increasing. T2DM is a multifactorial, polygenetic disease due to impaired insulin secretion and insulin resistance. Single nucleotide polymorphisms explain less than 20% of disease progression and heritability. Various studies have associated oxidative stress and environmental influence on development of T2DM. Hence, this study was designed with the objective of exploring the association of global DNA methylation and oxidative stress with glycemic control in T2DM patient. The study included 42 T2DM patients (35 to 70 yrs of age) without any complications and an equal number of matched healthy volunteers as controls. An equal number of matched T2DM patients with complication were also included in the study population. A twelve hour overnight fasting blood sample was used to determine HOMA-IR (marker for insulin resistance), Glycated Hb (marker for glycemic control), oxidant load (Ferrous oxidation xylenol orange--FOX2 assay), Total antioxidant capacity (Ferric reducing antioxidant power--FRAP assay) to assertion oxidative stress. Global DNA methylation (epigenetic changes) was estimated by ELISA kits after extraction of DNA by Magnapure (Roche diagnostics). An increased HOMAIR, oxidant load and DNA methylation was observed in T2DM patients with complications as compared to T2DM patients without any complications. Similar trend was observed among T2DM patients and controls. DNA methylation correlated with oxidative stress and HOMA-IR positively. The study reveals the positive association of DNA methylation with HOMA-IR and oxidative stress, implicating a key role of epigenetics in disease progression and development of complication in T2DM.
ISSN:0970-1915
0974-0422