Physicochemical and bioinformatic characterization of Oreochromis niloticus vitellogenin as an endocrine disruption biomarker

Aquatic biota is increasingly being exposed to chemical pollutants due to human activities and the relationship between the level of environmental pollution and fish reproduction is a continuously ongoing issue. The vitellogenin (Vtg) protein synthesis can be induced in the liver of juvenile and mal...

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Published in:Ecotoxicology (London) 2023, Vol.32 (1), p.12-24
Main Authors: Souza, Tugstênio L., de Morais, Tobias P., Neto, Francisco Filipak, Opuskevitch, Iracema, Ferreira, Fernando C. A. S., Randi, Marco Antônio Ferreira, de Oliveira Ribeiro, Ciro Alberto, de Souza, Claudemir, Prodocimo, Maritana Mela
Format: Article
Language:English
Subjects:
Animals
Biomarkers - metabolism
Cichlids
Earth and Environmental Science
Ecology
Ecotoxicology
Environment
Environmental Management
Estradiol
Ethinyl estradiol
Ethinyl Estradiol - toxicity
Ethylenediaminetetraacetic acid
Fish Proteins
Fishes
Male
Phylogeny
Protein biosynthesis
Vitellogenins - metabolism
Water Pollutants, Chemical - analysis
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Summary:Aquatic biota is increasingly being exposed to chemical pollutants due to human activities and the relationship between the level of environmental pollution and fish reproduction is a continuously ongoing issue. The vitellogenin (Vtg) protein synthesis can be induced in the liver of juvenile and male fish after stimulation of the estrogen receptor and therefore, Vtg has been used as a biomarker of xenoestrogen exposure in several fish species. The current study reported the first physicochemical characterization of Vtg from Oreochromis niloticus . Adult male fish were exposed to 17α-ethinylestradiol for Vtg induction. Purified vitellogenin from plasma showed low stability at 25 and 4 °C in saline conditions, and good stability in acidic (low pH) or in heated conditions. The 3D modeling provided useful information on the structure of O. niloticus Vtg showing conserved structural features. According to bioinformatics and experimental results, there are important structural differences between the two chemical forms of Vtg (VtgAb and VtgC) in a phylogenetic context. The present results add information about the development of ecotoxicological immunoassays to study the endocrine disruption in O. niloticus improving the Vtg performance as a biomarker of reproduction in fish.
ISSN:0963-9292
1573-3017
DOI:10.1007/s10646-022-02612-9