THP-1 cells transduced with CD16A utilize Fc[gamma] receptor I and III in the phagocytosis of IgG-sensitized human erythrocytes and platelets

Fc gamma receptors (Fc[gamma]Rs) are critical effector receptors for immunoglobulin G (IgG) antibodies. On macrophages, Fc[gamma]Rs mediate multiple effector functions, including phagocytosis, but the individual contribution of specific Fc[gamma]Rs to phagocytosis has not been fully characterized. P...

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Bibliographic Details
Published in:PloS one 2022-12, Vol.17 (12), p.e0278365
Main Authors: Gil Gonzalez, Lazaro, Fernandez-Marrero, Yuniel, Norris, Peter Alan Albert, Tawhidi, Zoya, Shan, Yuexin, Cruz-Leal, Yoelys, Won, Kevin Doyoon, Frias-Boligan, Kayluz, Branch, Donald R, Lazarus, Alan H
Format: Article
Language:English
Subjects:
Analysis
Fc receptors
Health aspects
Immunoglobulin G
Macrophages
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Summary:Fc gamma receptors (Fc[gamma]Rs) are critical effector receptors for immunoglobulin G (IgG) antibodies. On macrophages, Fc[gamma]Rs mediate multiple effector functions, including phagocytosis, but the individual contribution of specific Fc[gamma]Rs to phagocytosis has not been fully characterized. Primary human macrophage populations, such as splenic macrophages, can express Fc[gamma]RI, Fc[gamma]RIIA, and Fc[gamma]RIIIA. However, there is currently no widely available monocyte or macrophage cell line expressing all these receptors. Common sources of monocytes for differentiation into macrophages, such as human peripheral blood monocytes and the monocytic leukemia cell line THP-1, generally lack the expression of Fc[gamma]RIIIA (CD16A). Here, we utilized a lentiviral system to generate THP-1 cells stably expressing human Fc[gamma]RIIIA (CD16F158). THP-1-CD16A cells treated with phorbol 12-myristate 13-acetate for 24 hours phagocytosed anti-D-opsonized human red blood cells primarily utilizing Fc[gamma]RI with a lesser but significant contribution of IIIA while phagocytosis of antibody-opsonized human platelets equally utilized Fc[gamma]RI and Fc[gamma] IIIA. Despite the well-known ability of Fc[gamma]RIIA to bind IgG in cell free systems, this receptor did not appear to be involved in either RBC or platelet phagocytosis. These transgenic cells may constitute a valuable tool for studying macrophage Fc[gamma]R utilization and function.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0278365