Comparison of Two Staining Methods for Pollen Viability Studies in Sugarcane

The present work aimed to compare two staining methods for pollen viability evaluation in sugarcane. Pollen from four sugarcane genotypes were collected at three different times (6.00, 8.00 and 9.00 a.m.) and tested for viability using two staining methods (iodine and lactophenol blue). Three anther...

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Bibliographic Details
Published in:Sugar tech : an international journal of sugar crops & related industries 2013-03, Vol.15 (1), p.103-107
Main Authors: Melloni, Maria Letícia G., Scarpari, Maximiliano S., de Mendonça, Jeremias R., Perecin, Dilermando, de Andrade Landell, Marcos G., Pinto, Luciana R.
Format: Article
Language:English
Subjects:
Agriculture
Analysis
Biomedical and Life Sciences
Germination
Life Sciences
Methods
Short Communication
Sugarcane
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Summary:The present work aimed to compare two staining methods for pollen viability evaluation in sugarcane. Pollen from four sugarcane genotypes were collected at three different times (6.00, 8.00 and 9.00 a.m.) and tested for viability using two staining methods (iodine and lactophenol blue). Three anthers, of each genotype were crushed in a glass slide with a drop of the respective stain (iodine 0.1 N and lactophenol blue). The percentage of pollen viability was obtained with an optic microscope (250×) and compared with the pollen germination at culture media where one raquis of each genotype was gentle shaken in a petridish. Three replicates (petri dishes) was performed for each genotype which were maintained at the temperature of 25 °C and air humidity around 95 % for 30 min. The factors (staining methods, genotypes and times) and their interactions were evaluated by the analysis of variance, F test ( P  
ISSN:0972-1525
0974-0740
DOI:10.1007/s12355-012-0185-6