Molecular Diagnostic of Both Brown and Orange Sugarcane Rust and Evaluation of Sugarcane Brown Rust Resistance in Tucuman, Argentina, Using Molecular Markers Associated with Bru1 a Broad-Range Resistance Allele

Brown rust ( Puccinia melanocephala ) and orange rust ( P. kuehnii ) cause important yield loss in global sugarcane production. Due to the difficulties of distinguishing between the two diseases to the naked eye, it is essential to use molecular techniques for an accurate rust diagnosis. A major gen...

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Bibliographic Details
Published in:Sugar tech : an international journal of sugar crops & related industries 2016-08, Vol.18 (4), p.414-419
Main Authors: Racedo, J., Perera, M. F., Bertani, R., Funes, C., González, V., Cuenya, M. I., D′Hont, A., Welin, B., Castagnaro, A. P.
Format: Article
Language:English
Subjects:
Agriculture
Analysis
Biomedical and Life Sciences
Life Sciences
Research Article
Sugarcane
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Summary:Brown rust ( Puccinia melanocephala ) and orange rust ( P. kuehnii ) cause important yield loss in global sugarcane production. Due to the difficulties of distinguishing between the two diseases to the naked eye, it is essential to use molecular techniques for an accurate rust diagnosis. A major gene, Bru 1, which confers resistance to a broad spectrum of P. melanocephala strains in different parts of the world, has been described, and molecular markers closely associated with this allele have been developed. The aims of the present study were: (1) to optimise a PCR-based method to diagnose and characterise the causal agent of both rusts in Tucumán; (2) to determine the usefulness of the Bru 1 gene in the Sugarcane Breeding Program of “Estación Experimental Agroindustrial Obispo Colombres” (EEAOC) by studying its association with resistant and susceptible phenotypes; and (3) to assess the frequency of the Bru 1 allele in the sugarcane germplasm of the EEAOC. Conditions for both rust diagnoses were optimised. Out of 129 genotypes evaluated to study the usefulness of the Bru 1 allele, 49 were found to be resistant to brown rust, but only eight of these resistant genotypes were positive for the Bru 1 allele. Also frequency of appearance of the Bru 1 allele was analysed in 191 sugarcane accessions of the EEAOC germplasm, and its presence was detected in only 7 % of the genotypes evaluated. In conclusion, although results showed that Bru 1 markers enable positive selection of this character, additional source(s) of resistance are available in the EEAOC Sugarcane Breeding Program.
ISSN:0972-1525
0974-0740
0972-1525
DOI:10.1007/s12355-015-0404-z