Boronate affinity magnetic nanoparticles with hyperbranched polymer brushes for the adsorption of cis-diol biomolecules

A boronate-modified magnetic affinity sorbent was prepared by adopting hyperbranched polyethyleneimine as the scaffold to amplify initiator sites. 3-Acrylamidophenylboronic acid was employed as monomer to proceed in situ free-radical polymerization on magnetite (Fe 3 O 4 ) nanoparticles. Due to the...

Full description

Saved in:
Bibliographic Details
Published in:Mikrochimica acta (1966) 2019-10, Vol.186 (10), p.683, Article 683
Main Authors: He, Maofang, Wei, Yinmao, Wang, Rong, Wang, Chunyang, Zhang, Bo, Han, Lu
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Analytical Chemistry
Biochemistry
Biotechnology
Boronic Acids - chemistry
Catecholamines
Catecholamines - urine
Catechols - chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatography, Affinity
Chromatography, High Pressure Liquid
Dopamine - chemistry
Egg White - chemistry
Epinephrine - chemistry
Glycols
Glycoproteins
Glycoproteins - isolation & purification
Horseradish Peroxidase - chemistry
Humans
Magnetite Nanoparticles - chemistry
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Norepinephrine - chemistry
Original Paper
Ovalbumin - chemistry
Polyethyleneimine - chemistry
Polymer industry
Polymerization
Polymers
Solid Phase Extraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A boronate-modified magnetic affinity sorbent was prepared by adopting hyperbranched polyethyleneimine as the scaffold to amplify initiator sites. 3-Acrylamidophenylboronic acid was employed as monomer to proceed in situ free-radical polymerization on magnetite (Fe 3 O 4 ) nanoparticles. Due to the improved density of boronic acid polymers, the adsorbent exhibited high adsorption capacity, typically (134 ± 8) μg mg −1 for dopamine, (92 ± 7) μg mg −1 for catechol, (363 ± 14) μg mg −1 for ovalbumin and (464 ± 22) μg mg −1 for horseradish peroxidase. These capacities are much higher than those of other adsorbents. The sorbent was applied to the enrichment of catecholamines from spiked human urine. Owing to the high adsorption capacity, only 1.0 mg of adsorbent was sufficient to eliminate the interferences and enrich the targets (dopamine, norepinephrine and epinephrine) within 5 min. They were quantified by HPLC. The recoveries from spiked samples range between 83.5% ~106%, with relative standard deviations of 3.2 ~ 9.4% ( n  = 5). The separation of glycoproteins from egg white was also accomplished prior to their analysis by PAGE. In the authors’ perception, this approach is promising in that the density of functional groups on the adsorbent is strongly increased. Graphical abstract The preparation routine of boronate affinity magnetic adsorbent (Fe 3 O 4 @HpAAPBA). The adsorbent is used for the magnetic solid phase extraction of cis -dol compounds from real sample.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-019-3785-y