Tyrosine hydroxylase and [[beta].sub.2]-adrenergic receptor expression in leukocytes of spontaneously hypertensive rats: putative peripheral markers of central sympathetic activity

The sympathetic nervous system (SNS) plays a fundamental role in the pathophysiology of cardiovascular diseases, including primary arterial hypertension. In this study, we aimed to investigate whether the expression of the rate-limiting enzyme in catecholamine synthesis, tyrosine hydroxylase (TH), a...

Full description

Saved in:
Bibliographic Details
Published in:Brazilian journal of medical and biological research 2020-12, Vol.53 (12)
Main Authors: Nisimura, L.M, Bousquet, P, Muccillo, F, Tibirica, E, Garzoni, L.R
Format: Article
Language:English
Subjects:
Clonidine
Hydroxylases
Hypertension
Phenols (Class of compounds)
Tyrosine
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sympathetic nervous system (SNS) plays a fundamental role in the pathophysiology of cardiovascular diseases, including primary arterial hypertension. In this study, we aimed to investigate whether the expression of the rate-limiting enzyme in catecholamine synthesis, tyrosine hydroxylase (TH), and the [[beta].sub.2]-adrenergic receptor ([[beta].sub.2]-AR) in immune cells from peripheral blood, reflect central SNS activity in spontaneously hypertensive rats (SHR). TH expression in the lower brainstem and adrenal glands and [[beta].sub.2]-AR expression in the lower brainstem were analyzed by western blot analyses. In the leukocytes, TH and [[beta].sub.2]-AR expression was evaluated by flow cytometry before and after chronic treatment with the centrally-acting sympathoinhibitory drug clonidine. Western blot analyses showed increased TH and [[beta].sub.2]-AR expression in the lower brainstem and increased TH in adrenal glands from SHR compared to normotensive Wistar Kyoto rats (WKY). Lower brainstem from SHR treated with clonidine presented reduced TH and [[beta].sub.2]-AR levels, and adrenal glands had decreased TH expression compared to SHR treated with vehicle. Flow cytometry showed that the percentage of leukocytes that express [[beta].sub.2]-AR is higher in SHR than in WKY. However, the percentage of leukocytes that expressed TH was higher in WKY than in SHR. Moreover, chronic treatment with clonidine normalized the levels of TH and [[beta].sub.2]-AR in leukocytes from SHR to similar levels of those of WKY. Our study demonstrated that the percentage of leukocytes expressing TH and [[beta].sub.2]-AR was altered in arterial hypertension and can be modulated by central sympathetic inhibition with clonidine treatment. Key words: Brain stem; Sympathetic nervous system; Leukocytes; Spontaneously hypertensive rats; Clonidine
ISSN:0100-879X
1414-431X
DOI:10.1590/1414-431X20209615