Eucalyptol Inhibits Amyloid-[beta]-Induced Barrier Dysfunction in Glucose-Exposed Retinal Pigment Epithelial Cells and Diabetic Eyes

Hyperglycemia elicits tight junction disruption and blood-retinal barrier breakdown, resulting in diabetes-associated vison loss. Eucalyptol is a natural compound found in eucalyptus oil with diverse bioactivities. This study evaluated that eucalyptol ameliorated tight junctions and retinal barrier...

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Bibliographic Details
Published in:Antioxidants 2020-10, Vol.9 (10)
Main Authors: Kim, Dong Yeon, Kang, Min-Kyung, Lee, Eun-Jung, Kim, Yun-Ho, Oh, Hyeongjoo, Kim, Soo-Il, Oh, Su Yeon, Na, Woojin, Kang, Young-Hee
Format: Article
Language:English
Subjects:
Amyloid beta-protein
Diabetic retinopathy
Epithelial cells
Health aspects
Physiological aspects
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Summary:Hyperglycemia elicits tight junction disruption and blood-retinal barrier breakdown, resulting in diabetes-associated vison loss. Eucalyptol is a natural compound found in eucalyptus oil with diverse bioactivities. This study evaluated that eucalyptol ameliorated tight junctions and retinal barrier function in glucose/amyloid-[beta] (A[beta])-exposed human retinal pigment epithelial (RPE) cells and in db/db mouse eyes. RPE cells were cultured in media containing 33 mM glucose or 5 [micro]M A[beta] for 4 days in the presence of 1-20 [micro]M eucalyptol. The in vivo animal study employed db/db mice orally administrated with 10 mg/kg eucalyptol. Nontoxic eucalyptol inhibited the A[beta] induction in glucose-loaded RPE cells and diabetic mouse eyes. Eucalyptol reversed the induction of tight junction-associated proteins of ZO-1, occludin-1 and matrix metalloproteinases in glucoseor A[beta]-exposed RPE cells and in diabetic eyes, accompanying inhibition of RPE detachment from Bruch's membrane. Adding eucalyptol to glucose- or A[beta]-loaded RPE cells, and diabetic mouse eyes reciprocally reversed induction/activation of apoptosis-related bcl-2, bax, cytochrome C/Apaf-1 and caspases. Eucalyptol attenuated the generation of reactive oxygen species and the induction of receptor for advanced glycation end products in A[beta]-exposed RPE cells and diabetic eyes. Eucalyptol may ameliorate RPE barrier dysfunction in diabetic eyes through counteracting A[beta]-mediated oxidative stress-induced RPE cell apoptosis.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox9101000