Staphylococcal slime layers and biofilm from different origins/Slime layer e biofilme de Staphylococcus de diferentes origens

The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of cap...

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Bibliographic Details
Published in:Ciência rural 2019-05, Vol.49 (5)
Main Authors: Cunha, Rodrigo Casquero, da Rosa, Michelle Dias Hornes, da Silva, Cleomar, Santos, Francisco Denis Souza, Leite, Fabio Pereira Leivas
Format: Article
Language:English
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Summary:The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of capsule and biofilm, which contribute to its infectivity. The objective of this study was to observe the production of slime layer and formation of biofilm, which are related to the resistance to antimicrobial agents and presence of icaA and icaD genes, in 41 isolates of Staphylococcus spp. from different origins, provided by the Universidade Federal de Pelotas (UFPEL), Laboratorio Regional de Diagnostico (LRD). Strains of Staphylococcus spp. were cultivated in Congo red agar for capsule detection. Biofilm formation was detected using the 96-well microplate testing. Antimicrobial susceptibility testing was performed using the plate diffusion method. Part of the analyzed samples produced slime layer (36.6%) andformed biofilm (17.1%). However, six of those that formed biofilms were susceptible to the eight antibiotics tested in the antibiogram. In tests to determine the minimum bactericidal and inhibitory concentrations, gentamicin resistance of biofilm-forming strains was greater than that of non-forming strains. Ampicillin was the least effective antimicrobial drug (51%), followed by tetracycline (71%), neomycin (73%), and erythromycin (73%). Some isolates presented the icaA (6) and icaD (11) genes. Therefore, we suggested that the origin of an isolate can determine its expression of virulence factor and resistance to certain antibiotics. Key words: Staphylococcus, biofilm, slime layer. O genero Staphylococcus abrange algumas das bacterias patogenicas mais importantes tanto para humanos como para animais. Ele e responsavel pela mastite bovina e otite canina, alem de estar presente na microbiota de animais e como contaminante em alimentos. Sua patogenese esta relacionada a formacao de capsula e biofilme, que contribuem para sua infectividade. O objetivo deste estudo foi observar a producao de slime layer e a formacao de biofilme, que estao relacionados a resistencia a antibicrobianos e a presenca dos genes icaA e icaD, em 41 isolados de Staphylococcus spp. de diferentes origens fornecidos pelo Laboratorio Regional de Diagnostico (LRD) da Universidade Federal de Pelotas (UFPEL). Os isolados de Staphylococcus spp. foram cultivados em agar vermelho do Congo para detecc
ISSN:0103-8478
1678-4596
DOI:10.1590/0103-8478cr20180783