Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?/ ?El Trasplante de Celulas Madre Neuroectodermicas Restaura la Regeneracion Neural y las Funciones Locomotoras en el Modelo de Rata con Lesion de la Medula Espinal Comprimida?

The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each). Compresse...

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Published in:International journal of morphology 2019-03, Vol.37 (1), p.349
Main Authors: Al-Karim, Saleh, Ramadan, Wafaa S, Abdel-Hamid, Ghada A, Qudsi, Fatma Al
Format: Article
Language:English
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Summary:The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each). Compressed spinal cord injured (CSCI) were forty rats which subdivided equally into: untreated, treated by neuroectodermal stem cells (NESCs). After four weeks, all rats in different groups were scarified, samples were taken from central, cranial, and caudal to the site of spinal cord injury. Specimens were prepared for light and electron microscopic examination. The number of remyelinated axons in central, cranial and caudal regions to the injured spinal cord after transplantation of NESCs was counted. The open field test assessed the locomotor function. Results revealed that compressed spinal cord injury resulted in loss and degeneration of numerous nerve fibers, myelin splitting and degeneration of mitochondria. Four weeks after transplantation of NESCs regenerated axons were noticed in cranial and central sites, while degenerate axons were noticed caudal to the lesion. Number of remyelinated axons was significantly increased in both central and cranial to the site of spinal cord injury in comparison with caudal region which had the least number of remyelinated axons. Transplantation of NESCs improved significantly the locomotor functional activity In conclusion, neuroectodermal stem cells transplantation ameliorated the histopathological and ultrastructural changes, and improved the functional locomotor activity in CSCI rat. KEY WORDS: Neuroectodermal; Stem cells; Spinal cord; Injury; Regeneration; Rat. RESUMEN: El objetivo de este estudio fue determinar el posible efecto regenerativo de las celulas madre neuroectodermicas en la funcion ultraestructural y locomotora de una lesion comprimida en la medula espinal en un modelo de rata. Cuarenta ratas macho se dividieron en grupos control y sham (20 ratas en cada grupo). La medula espinal lesionada (CSCI) tenia cuarenta ratas que se subdividieron de igual forma en los siguientes grupos: no tratadas, tratadas con celulas madre neuroectodermicas (NESCs). Al termino de cuatro semanas, todas las ratas en los diferentes grupos fueron escarificadas, se tomaron muestras de las areas central, craneal y caudal en relacion al sitio de la lesion de la medula espinal. Las muestras fueron preparadas para examen microscopico de luz y
ISSN:0717-9367
0717-9502