Fabrication and evaluation of a [gamma]-PGA-based self-assembly transferrin receptor-targeting anticancer drug carrier

Background: cis-Dichlorodiamineplatinum (CDDP) was one of the most common used drugs in clinic for cancer treatment. However, CDDP caused a variety of side effects. The abundant carboxyl groups on the surface of poly glutamic acid (PGA) could be modified with various kinds of targeted ligands. PGA d...

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Bibliographic Details
Published in:International journal of nanomedicine 2018-01, Vol.13, p.7873
Main Authors: Zhang, Li, Zhu, Xiaoyu, Wu, Shijia, Chen, Yazhou, Tan, Shiming, Liu, Yingjie, Jiang, Wenzheng, Huang, Jing
Format: Article
Language:English
Subjects:
Cancer
Drug delivery systems
Drugs
Electron microscopy
Fluorescence
Fluorescence microscopy
Glutamate
Health aspects
Microscopy
Peptides
Polyamides
Toxicity
Transferrin
Tumors
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Summary:Background: cis-Dichlorodiamineplatinum (CDDP) was one of the most common used drugs in clinic for cancer treatment. However, CDDP caused a variety of side effects. The abundant carboxyl groups on the surface of poly glutamic acid (PGA) could be modified with various kinds of targeted ligands. PGA delivery system loaded CDDP for cancer therapies possesses potential to overcome the side effects. Materials and methods: In this study, we constructed a safe and efficient anticancer drug delivery system PGA--Asp--maleimide--cisplatin--peptide complex (PAMCP), which was loaded with CDDP and conjugated with the transferrin receptor (TFR)-targeting peptide through a maleimide functional linker. The size of PAMCP was identified by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Fluorescence microscopy and flow cytometry methods were used to detect the cell targeting ability in vitro. The MTT assay was used to detect targeted toxicity in vitro. The in vivo acute toxicity was tested in Kun Ming (KM) mice. The tumor suppression activity and drug distribution was analyzed in nude mice bearing with HeLa tumor cells. Results: The nano-size was 110+28 nm detected with TEM and 89+18 nm detected with DLS, respectively. Fluorescence microscopy and flow cytometry methods indicated that PAMCP possessed excellent cell targeting ability in vitro. The MTT assay suggested that PAMCP was excellent for targeted toxicity. The acute in vivo toxicity study revealed that the body mass index and serum index in the PAMCP-treated group were superior to those in the CDDP-treated group (P
ISSN:1178-2013
1178-2013
DOI:10.2147/IJN.S181121