Effects of adipokine zinc-2-glycoprotein on adipose tissue metabolism after dexamethasone treatment

Zinc-[alpha]2-glycoprotein (ZAG) has been demonstrated to play a role in stimulating lipid mobilization under normal conditions. However, further studies are required to determine whether ZAG overexpression can alleviate the reduction in plasma lipid levels under stress conditions. In the present st...

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Bibliographic Details
Published in:Applied physiology, nutrition, and metabolism nutrition, and metabolism, 2019-01, Vol.44 (1), p.83
Main Authors: Qiao, Yu, Fan, Guoqiang, Guo, Jun, Gao, Shixing, Zhao, Ruqian, Yang, Xiaojing
Format: Article
Language:English
Subjects:
Dexamethasone
Genetic aspects
Glycoproteins
Health aspects
Lipid metabolism
Patient outcomes
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Summary:Zinc-[alpha]2-glycoprotein (ZAG) has been demonstrated to play a role in stimulating lipid mobilization under normal conditions. However, further studies are required to determine whether ZAG overexpression can alleviate the reduction in plasma lipid levels under stress conditions. In the present study, we investigated the effects of ZAG on lipometabolism in white adipose tissue (WAT) after dexamethasone (DEX) stimulation using C57BL/6 male mice as the experimental models. Transcript and protein levels of genes associated with the [beta]-adrenoreceptor ([beta]-AR)/cyclic adenosine monophosphate/protein kinase a (PKA) pathway, lipid mobilization, and energy metabolism were determined by quantitative real-time polymerase chain reaction and Western blotting. Plasma levels of nonesterified fatty acid (NEFA) were measured using an automatic biochemical analyzer. Results indicated that plasma NEFA levels were decreased in the DEX group, but NEFA levels were rescued by ZAG overexpression. ZAG overexpression resulted in the upregulation of [beta]3-AR and phosphorylated PKA protein relative to those of the DEX group. Analysis of lipometabolism showed that protein levels of phosphorylated hormone-sensitive lipase was reduced upon DEX treatment but were restored by ZAG overexpression. For energy metabolism, ZAG significantly upregulated the protein expression of carnitine palmitoyltransferase^ and cytochrome c oxidase subunit 1 relative to those of the DEX group. In conclusion, ZAG could alleviate DEX-induced decrease in plasma NEFA levels and this could be associated with the promoting lipid mobilization in WAT.Key words: ZAG, dexamethasone, NEFA, white adipose tissue, lipometabolism.Resume : La glycoproteine zinc-[alpha]2 (>) joue, etudes a l'appui, un role dans la stimulation de la mobilisation des lipides dans des conditions normales. Toutefois, il faut effectuer d'autres etudes pour verifier si la surexpression de ZAG peut attenuer la diminution du niveau plasmatique des lipides dans des conditions de stress. Dans la presente etude, on examine les effets de ZAG sur le lipometabolisme du tissu adipeux blanc (>) stimule a la dexamethasone (>) chez des souris C57BL/6 males en tant que modeles experimentaux. On determine la transcription et la concentration de genes associes a la voie de la [beta]-adrenorecepteur (>)/cyclique adenosine monophosphate/proteine kinase (>), la mobilisation des lipides et le metabolisme energ
ISSN:1715-5312
1715-5320
DOI:10.1139/apnm-2018-0165