Optimized acid hydrolysis of the polysaccharides from the seaweed Solieria filiformis P.W. Gabrielson para producao de bioetanol

The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily...

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Published in:Acta scientiarum. Biological sciences 2017-10, Vol.39 (4), p.423
Main Authors: Castro, George Meredite Cunha de, Benevides, Norma Maria Barros, Cabral, Maulori Curie, Souza Miranda, Rafael de, Filho, Eneas Gomes, Rocha, Maria Valderez Ponte, Araujo, Marjory Lima Holanda
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Language:English
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Summary:The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were [Y.sub.(P/S)] = 0.48 [+ or -] 0.02 g.[g.sup.-1], [P.sub.P] = 0.27 [+ or -] 0.04 g.[L.sup.-1].[h.sub.-1], [eta]= 94.1%, representing a 41% increase in bioethanol productivity. Therefore, S. filiformis was a promising renewable resource of polysaccharides easily hydrolyzed, generating a broth rich in fermentable monosaccharides for ethanol production. Keywords: iota-carrageenan, cellulose, galactose, kinetic parameters, fermentation, acclimation, sulfuric acid. As algas marinhas sao recursos naturais ricos em polissacarideos sulfatados e neutros. A especie de macroalga tropical utilizada neste estudo (Solieria filiformis) apresentou teores de carboidratos de 65,8% (m/m), proteinas de 9,6% (m/m), lipidios de 1,7% (m/m), umidade de 7,0% (m/m) e 15,9 % (m/m) de cinzas. A macroalga seca foi facilmente hidrolisada em condicoes brandas, na presenca de acido sulfurico 0,5 M, por 20 min, produzindo monossacarideos fermentaveis com uma eficiencia de hidrolise maxima de 63,21%. A galactose e a glicose presentes no hidrolisado foram fermentadas simultaneamente por Saccharomyces cerevisiae, apos aclimatacao da levedura cultivada em meio contendo apenas galactose como fonte de carbono. Os parametros cineticos da fermentacao do hidrolisado algaceo pela levedura aclimatada a galactose foram [Y.sub.(P/S)] = 0,48 [+ or -] 0,02 g.[g.sup.-1], [P.sub.P] = 0,27 [+ or -] 0,04 g.[L.sup.-1].[h.sup.-1], [eta] = 94,1%. Portanto, a macroalga S. filiformis se mostrou um recurso renovavel promissor como fonte de polissacarideos facilmente hidrolisados, gerando um meio nutritivo rico em glucose e galactose para a producao de etanol. Palavras-chave: iota-carragenana, celulose, galactose, parametros cineticos, fermentacao, aclimatacao, acido sulfurico.
ISSN:1679-9283
1807-863X
DOI:10.4025/actascibiolsci.v39i4.37227