Discovery of nitrateCPKNLP signalling in central nutrientgrowth networks

Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca[sup.2+] signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in...

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Bibliographic Details
Published in:Nature (London) 2017-05, Vol.545 (7654), p.311
Main Authors: Liu, Kun-hsiang, Niu, Yajie, Konishi, Mineko, Wu, Yue, Du, Hao, Sun Chung, Hoo, Li, Lei, Boudsocq, Marie, McCormack, Matthew, Maekawa, Shugo, Ishida, Tetsuya, Zhang, Chao, Shokat, Kevan, Yanagisawa, Shuichi, Sheen, Jen
Format: Article
Language:English
Subjects:
Genetic aspects
Nitrates
Plant growth
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Summary:Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca[sup.2+] signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca[sup.2+]-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca[sup.2+] signalling network integrates transcriptome and cellular metabolism with shootroot coordination and developmental plasticity in shaping organ biomass and architecture.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature22077