Genetic monitoring by rapd markers for repopulation programs of Salminus brasiliensis /Monitoramento genetico utilizando marcadores RAPD em programas de criacao em cativeiro de Dourado

The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of dourado samples from the middle Paranapanema river and from the broodstock used in the stock enhancement progra...

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Bibliographic Details
Published in:Acta scientiarum. Animal sciences 2013-04, Vol.35 (2), p.119
Main Authors: de Almeida, Fernanda Simoes, Lopes, Carla Ma, Orsi, Mario Luis, Sirol, Rodolfo Nardez, Sodre, Leda Maria Koelblinger
Format: Article
Language:Spanish
Subjects:
Aquaculture industry
Fishes
Genetic research
Hydroelectric power plants
Water-power
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Summary:The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of dourado samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most interpopulation genetic variation lay within stocks (83.9%) and not between them (16.1%). Moderate genetic differentiation ([F.sub.ST] = 0.16) was reported. Nevertheless, differentiation decreased when the four fry stocks were mixed and analyzed as a single population ([F.sub.ST] = 0.07). Instead of releasing each lot separately into the water, mixing specimens produced in the various fry stocks before releasing them in the river would be more feasible. The restocked population will have a genetic structure closer to natural populations. Keywords: dourado, genetic variability, hatchery stocks, fry stocks. Conservar a variabilidade genetica dos estoques de alevinos de peixes para suplementar populacoes naturais e uma prioridade. O objetivo deste estudo foi examinar a diversidade genetica por meio dos marcadores de RAPD de amostras de peixes reprodutores de dourado do medio Paranapanema (populacao natural) e alevinos usados no programa de repovoamento da estacao de aquicultura da UHE de Salto Grande. Foram analisados 19 primers de RAPD, que geraram 299 bandas, as quais foram utilizadas para as analises geneticas. A porcentagem de fragmentos polimorficos foi mais elevada na populacao natural do que nos estoques. A diversidade genetica tambem foi mais baixa nos alevinos. Os resultados da Amova mostraram que a maior parte de variacao genetica interpopulacional esta dentro dos estoques (83,9%) e nao entre eles (16,1%). Foi observada uma diferenciacao genetica moderada ([F.sub.ST] = 0.16). Esta diferenciacao diminuiu quando os quatro estoques de alevinos eram misturados e analisados como uma unica populacao ([F.sub.ST] = 0.07). Em vez de liberar cada lote separadamente no ambiente, seria melhor misturar todos os individuos produzidos nos varios est
ISSN:1806-2636
1807-8672
DOI:10.4025/actascianimsci.v35i2.15904