Inhibition of Fumonisin B.sub.1 Cytotoxicity by Nanosilicate Platelets during Mouse Embryo Development

Nanosilicate platelets (NSP), the form of natural silicate clay that was exfoliated from montmorillonite (MMT), is widely used as a feed additive for its high non-specific binding capacity with mycotoxins such as fumonisin B.sub.1 (FB.sub.1 ), and has been evaluated its safety for biomedical use inc...

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Bibliographic Details
Published in:PloS one 2014-11, Vol.9 (11)
Main Authors: Liao, Yu-Jing, Yang, Jenn-Rong, Chen, Shuen-Ei, Wu, Sing-Jhou, Huang, San-Yuan, Lin, Jiang-Jen, Chen, Lih-Ren, Tang, Pin-Chi
Format: Article
Language:English
Subjects:
Adsorption
Embryonic development
Gene expression
Lipids
Montmorillonite
Phosphates
Silicon compounds
Sphingosine
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Summary:Nanosilicate platelets (NSP), the form of natural silicate clay that was exfoliated from montmorillonite (MMT), is widely used as a feed additive for its high non-specific binding capacity with mycotoxins such as fumonisin B.sub.1 (FB.sub.1 ), and has been evaluated its safety for biomedical use including cytotoxicity, genotoxicity, and lethal dosage (LD). In the study, we further examined its toxicity on the development of CD1 mouse embryos and its capacity to prevent teratogenesis-induced by FB.sub.1 . In vitro cultures, NSP did not disturb the development and the quality of intact pre-implantation mouse embryos. Further, newborn mice from females consumed with NSP showed no abnormalities. NSP had an unexpected high adsorption capacity in vitro. In contrast to female mice consumed with FB.sub.1 only, a very low residual level of FB.sub.1 in the circulation, reduced incidence of neutral tube defects and significantly increased fetal weight were observed in the females consumed with FB.sub.1 and NSP, suggesting a high alleviation effect of NSP on FB.sub.1 in vivo. Furthermore, FB.sub.1 treatment disturbed the gene expression of sphingolipid metabolism enzymes (longevity assurance homolog 5, LASS 5; sphingosine kinase 1, Sphk1; sphingosine kinase 2, Sphk2; sphingosine 1- phosphate lyase, Sgpl1; sphingosine 1-phosphate phosphatase, Sgpp1) in the maternal liver, uterus, fetus, and placenta, but NSP administration reversed the perturbations. Based on these findings, we conclude that NSP is a feasible and effective agent for supplementary use in reducing the toxicity of FB.sub.1 to animals.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0112290