Mapping and characterization of [Rf.sub.5]: a new gene conditioning pollen fertility restoration in [A.sub.1] and [A.sub.2] cytoplasm in sorghum

With an aim to further characterize the cytoplasmic male sterility-fertility restoration system in sorghum, a major fertility restoration gene was mapped along with a second locus capable of partial restoration of pollen fertility. The major fertility restoration gene, [Rf.sub.5], was located on sor...

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Bibliographic Details
Published in:Theoretical and applied genetics 2011-08, Vol.123 (3), p.383
Main Authors: Jordan, D.R, Klein, R.R, Sakrewski, K.G, Henzell, R.G, Klein, P.E, Mace, E.S
Format: Article
Language:English
Subjects:
Analysis
Fertility
Genetic aspects
Sorghum
Online Access:Get full text
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Summary:With an aim to further characterize the cytoplasmic male sterility-fertility restoration system in sorghum, a major fertility restoration gene was mapped along with a second locus capable of partial restoration of pollen fertility. The major fertility restoration gene, [Rf.sub.5], was located on sorghum chromosome SBI-05, and was capable of restoring pollen fertility in both [A.sub.1] and [A.sub.2] male sterile cytoplasms. Depending on the restorer parent, mapping populations exhibited fertility restoration phenotypes that ranged from nearly bimodal distribution due to the action of [Rf.sub.5], to a more normalized distribution reflecting the action of [Rf.sub.5] and additional modifier/partial restoration genes. A second fertility restoration locus capable of partially restoring pollen fertility in [A.sub.1] cytoplasm was localized to chromosome SBI-04. Unlike [Rf.sub.5], this modifier/ partial restorer gene acting alone resulted in less than 10% seed set in both [A.sub.1] and [A.sub.2] cytoplasms, and modified the extent of restoration conditioned by the major restorer [Rf.sub.5] in [A.sub.1] cytoplasm. In examining the genomic regions spanning the [Rf.sub.5] locus, a cluster of pentatricopeptide gene family members with high homology to rice [Rf.sub.1] and sorghum [Rf.sub.2] were identified as potential candidates encoding [Rf.sub.5].
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-011-1591-y