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Identification of flavonoids in plants by HPLC
The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile...
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Published in: | Pharmaceutical chemistry journal 2011-04, Vol.45 (1), p.47-50 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile at a ratio of 80:20 (v/v), column temperature 30°C; absorption spectra were recorded over the range 190 – 400 nm. This chromatography system can be used for preliminary identification of flavonoids in plant materials. |
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ISSN: | 0091-150X 1573-9031 |
DOI: | 10.1007/s11094-011-0558-2 |