Identification of flavonoids in plants by HPLC

The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile...

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Bibliographic Details
Published in:Pharmaceutical chemistry journal 2011-04, Vol.45 (1), p.47-50
Main Authors: Moiseev, D. V., Buzuk, G. N., Shelyuto, V. L.
Format: Article
Language:English
Subjects:
Cholesterol
Chromatography
Isoflavones
Medicine
Nitriles
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
Phosphates
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Summary:The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile at a ratio of 80:20 (v/v), column temperature 30°C; absorption spectra were recorded over the range 190 – 400 nm. This chromatography system can be used for preliminary identification of flavonoids in plant materials.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-011-0558-2