Commercial ELISA[R] kit for detection of coproantigen and coproparasitological method in bovine livers with fascioliasis convicted/Kit comercial de ELISA[R] para a deteccao de coproantigenos e exame coproparasitologico em bovinos com figados condenados por fasciolose

The aim of this study was to compare a commercial ELISA kit for detection on coproantigen examination and fecal sedimentation using as gold standard inspection diagnosis of bovine livers at slaughter. In addition, we evaluated the correlation between the measured intensity of infection by counting e...

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Bibliographic Details
Published in:Ciência rural 2012-11, Vol.42 (11)
Main Authors: Bernardo, Cintia das Chagas, de Avelar, Barbara Rauta, Ignacchiti, Mariana Drummond Costa, Ma, Pereira, Maria Julia Salim
Format: Article
Language:Spanish
Subjects:
Analysis
Enzyme-linked immunosorbent assay
Fluke infections
Health aspects
Liver
Methods
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Summary:The aim of this study was to compare a commercial ELISA kit for detection on coproantigen examination and fecal sedimentation using as gold standard inspection diagnosis of bovine livers at slaughter. In addition, we evaluated the correlation between the measured intensity of infection by counting eggs in the feces and the parasites in bovine livers. Feces were collected and evaluated macroscopically of 81 cattle livers, 45 of which had livers condemned by liver flukes and in these livers parasites were counted. Two fractions of stool samples collected were separated and one stored in freezer for further ELISA and other one processed according to sedimentation technique for diagnosis Fasciola hepatica. The Spearman correlation and McNemar chi-square were used, adopting the significance of 5%. In eight bovine livers condemned by the characteristic lesions of fascioliasis parasite were not found. The stool examinations and ELISA testing for detection coproantigen, respectively, had sensitivity of 51.11% and 75.55%, specificity of 100% and 91,66%, predictive positive value was 100% and 91.89%, predictive negative value 62% and 75% and kappa 0.48 and 0.65. The results obtained by commercial[R] ELISA kit did not differ (P = 0,06) obtained at slaughterhouse, but the stool examinations differed (P < 0.0001) in the detection of the positive animals. The correlation between the number of parasites in the liver and the number of eggs in the feces was moderate (rs = 0.5757, P < 0.0001). The commercial ELISA kit[R] was more sensitive than the fecal test, althought this one shoud not be discarded because of their efficiency. Key words: Fasciola hepatica, bovine, immunodiagnosis. Objetivou-se com o presente estudo comparar um kit comercial de ELISA para a deteccao de coproantigenos e um exame coproparasitologico de sedimentacao utilizando-se como padrao ouro o diagnostico da inspecao de figados bovinos ao abate. Alem disso, avaliou-se a correlacao entre a intensidade parasitaria mensurada pela contagem de ovos nas fezes e a de parasitos ao abate. Foram coletadas as fezes e avaliados macroscopicamente os figados de 81 bovinos, dos quais 45 tiveram os figados condenados por fasciolose hepatica ao abate, nos quais se realizou a contagem dos parasitos. Duas fracoes de cada amostra de fezes coletadas foram separadas e uma delas foi armazenada em congelador para posterior realizacao do ELISA e a outra processada segundo uma tecnica de sedimentacao fecal para diagnostico de ov
ISSN:0103-8478
1678-4596