Controlled reperfusion for different durations in the treatment of ischemia–reperfusion injury of the rat ovary: evaluation of biochemical features, molecular gene expression, and histopathology

High numbers of proinflammatory cells (PMNLs), which are carried by the blood to ischemic tissue during reperfusion, are considered responsible for inducing the inflammatory response that occurs in ischemia–reperfusion (I/R) injury. Our objective was to determine the controlled reperfusion (CR) inte...

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Bibliographic Details
Published in:Canadian journal of physiology and pharmacology 2015-04, Vol.93 (4), p.269-274
Main Authors: Yapca, Omer Erkan, Kumbasar, Serkan, Salman, Suleyman, Yarali, Oguzhan, Sener, Ebru, Mammadov, Renad, Tekin, Yesim Bayoglu, Aksoy, Aysenur, Albayrak, Abdulmecit, Cetin, Nihal
Format: Article
Language:English
Subjects:
Animals
antioxidants
antioxydants
Biochemistry
Cell Adhesion
COX-1
COX-2
Cyclooxygenase 1 - genetics
Cyclooxygenase 1 - metabolism
Cyclooxygenase 2 - genetics
Cyclooxygenase 2 - metabolism
Edema - etiology
Edema - prevention & control
Female
Gene expression
Gene Expression Regulation, Enzymologic
Genetic aspects
Genetic research
Health aspects
hemorrhage
Hemorrhage - etiology
Hemorrhage - prevention & control
hémorragie
Ischemia
Membrane Proteins - genetics
Membrane Proteins - metabolism
Neutrophil Infiltration
Neutrophils - immunology
Neutrophils - metabolism
Neutrophils - pathology
Oophoritis - immunology
Oophoritis - metabolism
Oophoritis - pathology
Oophoritis - prevention & control
Ovaries
Ovary - blood supply
Ovary - immunology
Ovary - metabolism
Ovary - pathology
PMN
PMNL
Rats, Wistar
Reperfusion
Reperfusion Injury - immunology
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Reproductive system
Rodents
Time Factors
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Summary:High numbers of proinflammatory cells (PMNLs), which are carried by the blood to ischemic tissue during reperfusion, are considered responsible for inducing the inflammatory response that occurs in ischemia–reperfusion (I/R) injury. Our objective was to determine the controlled reperfusion (CR) interval duration (CRID) that would minimize the injury caused by the PMNLs that infiltrate ischemic tissue. Animal groups were divided into the following groups: Sham group, ovarian I/R group (OIR), and ovarian ischemia controlled-reperfusion groups OICR-1, OICR-2, OICR-3, OICR-4, OICR-5, OICR-6, which had their ovarian artery opened and then closed for 10, 8, 6, 4, 2, or 1 s, respectively. The results show that the COX-2 activity and the gene expression decreased while the COX-1 activity and the gene expression were found to be increased in parallel to the shortening of the period in CRID. From the histopathological examinations, the findings of hemorrhage, edema, congested vascular structures, degenerated cells, and migration and adhesion of PMNLs were scaled as follows: Sham group < OICR-6 < OICR-5 < OICR-4 < OICR-3 < OICR-2 < OICR-1. The results from the histopathological assessments were consistent with the molecular and biochemical findings. In conclusion, our findings suggest that increased COX-2 activity plays a role in I/R injury of the rat ovary, and that controlled reperfusion for 3, 2, or 1 s following 2 h of ischemia may attenuate the effects of I/R injury.
ISSN:0008-4212
1205-7541
DOI:10.1139/cjpp-2014-0359