Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study

A collaborative study was conducted for the determination of paralytic shellfish poisoning (PSP) toxins in shellfish. The method used liquid chromatography with fluorescence detection after prechromatographic oxidation of the toxins with hydrogen peroxide and periodate. The PSP toxins studied were s...

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Bibliographic Details
Published in:Journal of AOAC International 2005-11, Vol.88 (6), p.1714-1732
Main Authors: Lawrence, J.F, Niedzwiadek, B, Menard, C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Assay
Bivalvia
Chemistry Techniques, Analytical - methods
Chromatography - methods
Chromatography, Ion Exchange
Chromatography, Liquid - methods
clams
cooperative research
Fish and seafood industries
fluorescence
Fluorescence spectroscopy
Food
food analysis
food contamination
Food industries
food intoxication
food pathogens
Fundamental and applied biological sciences. Psychology
General aspects
gonyautoxins
high performance liquid chromatography
Liquid chromatography
Marine Toxins - analysis
Marine Toxins - chemistry
Methods
Methods of analysis, processing and quality control, regulation, standards
Mice
Ostreidae
Oxidants
Oxygen - metabolism
oysters
paralytic shellfish poisoning
Pectinidae
quantitative analysis
Reproducibility of Results
Saxitoxin - analogs & derivatives
Saxitoxin - analysis
saxitoxins
scallops
Shellfish
Spectrometry, Fluorescence - methods
Testing
Time Factors
Toxins, Biological
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Description
Summary:A collaborative study was conducted for the determination of paralytic shellfish poisoning (PSP) toxins in shellfish. The method used liquid chromatography with fluorescence detection after prechromatographic oxidation of the toxins with hydrogen peroxide and periodate. The PSP toxins studied were saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins 2 and 3 (GTX2,3; together), gonyautoxins 1 and 4 (GTX1,4; together), decarbamoyl saxitoxin (dcSTX), B-1 (GTX5), C-1 and C-2 (C1,2; together), and C-3 and C-4 (C3,4; together). B-2 (GTX6) toxin was also included, but for qualitative identification only. Mussels, both blank and naturally contaminated, were mixed and homogenized to provide a variety of PSP toxin mixtures and concentration levels. The same procedure was followed with clams, oysters, and scallops. Twenty-one test samples in total were sent to 21 collaborators who agreed to participate in the study. Results were obtained from 18 laboratories representing 14 different countries. It is recommended that the method be adopted First Action by AOAC INTERNATIONAL.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/88.6.1714