Marker Assisted Gene Pyramiding

Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a well-adapted pop...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-06, Vol.15 (6), p.e0234088
Main Authors: Jamaloddin, Md, Durga Rani, Ch. V, Swathi, G, Anuradha, Ch, Vanisri, S, Rajan, C. P. D, Krishnam Raju, S, Bhuvaneshwari, V, Jagadeeswar, R, Laha, G. S, Prasad, M. S, Satyanarayana, P. V, Cheralu, C, Rajani, G, Ramprasad, E, Sravanthi, P, Arun Prem Kumar, N, Aruna Kumari, K, Yamini, K. N, Mahesh, D, Sanjeev Rao, D, Sundaram, R. M, Madhav, M. Sheshu
Format: Article
Language:English
Subjects:
Bacterial plant diseases
Botanical research
Disease resistance (Plants)
Genetic aspects
Genotypes
Identification and classification
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacterial blight (BB) and fungal blast diseases are the major biotic constraints that limit rice productivity. To sustain yield improvement in rice, it is necessary to developed yield potential of the rice varieties by incorporation of biotic stress resistance genes. Tellahamsa is a well-adapted popular high yielding rice variety in Telangana state, India. However, the variety is highly susceptible to BB and blast. In this study, simultaneous stepwise transfer of genes through marker-assisted backcross breeding (MABB) strategy was used to introgress two major BB (Xa21 and xa13) and two major blast resistance genes (Pi54 and Pi1) into Tellahamsa. In each generation (from F.sub.1 to ICF.sub.3) foreground selection was done using gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13), Pi54MAS (Pi54) and RM224 (Pi1). Two independent BC.sub.2 F.sub.1 lines of Tellahamsa/ISM (Cross-I) and Tellahamsa/NLR145 (Cross-II) possessing 92% and 94% recurrent parent genome (RPG) respectively were intercrossed to develop ICF1-ICF.sub.3 generations. These gene pyramided lines were evaluated for key agro-morphological traits, quality, and resistance against blast at three different hotspot locations as well as BB at two locations. Two ICF.sub.3 gene pyramided lines viz., TH-625-159 and TH-625-491 possessing four genes exhibited a high level of resistance to BB and blast. In the future, these improved Tellahamsa lines could be developed as mega varieties for different agro-climatic zones and also as potential donors for different pre-breeding rice research.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0234088