Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1992-10, Vol.258 (5083), p.818-821
Main Authors: Kallioniemi, Anne, Olli-P. Kallioniemi, Sudar, Damir, Rutovitz, Denis, Gray, Joe W., Waldman, Fred, Pinkel, Dan
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cancer cells
Cell lines
Chromosome Mapping
Chromosomes
Comparative genomic hybridization
cytogenetics
DNA
DNA Probes
DNA, Neoplasm - genetics
Female
Fluorescein-5-isothiocyanate
Fluorescence
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Gene Amplification
Gene Deletion
General aspects
Genetic aspects
Genetic loci
Genomes
Human cytogenetics
Humans
hybridizations
In Situ Hybridization, Fluorescence
Male
Metaphase
Methods
Molecular and cellular biology
Multidisciplinary Sciences
Mutation
Neoplasms - genetics
Nucleic Acid Hybridization
Oncogenes
Polymorphism, Restriction Fragment Length
Rhodamines
Science & Technology
Science & Technology - Other Topics
Tumor cell line
Tumor Cells, Cultured
Tumors
tumours
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Description
Summary:Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1359641