novo transcriptome sequencing and sequence analysis of the malaria vector Anopheles sinensis (Diptera: Culicidae)

BACKGROUND: Anopheles sinensis is the major malaria vector in China and Southeast Asia. Vector control is one of the most effective measures to prevent malaria transmission. However, there is little transcriptome information available for the malaria vector. To better understand the biological basis...

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Bibliographic Details
Published in:Parasites & vectors 2014, Vol.7 (1)
Main Authors: Chen, Bin, Zhang, Yu-Juan, He, Zhengbo, Li, Wanshun, Si, Fengling, Tang, Yao, He, Qiyi, Qiao, Liang, Yan, Zhentian, Fu, Wenbo, Che, Yanfei
Format: Article
Language:English
Subjects:
adults
Anopheles sinensis
chromosome mapping
complementary DNA
data collection
eggs
genetic markers
genomics
insect vectors
larvae
malaria
microsatellite repeats
population genetics
pupae
RNA
sequence analysis
transcriptome
transcriptomics
unigenes
vector control
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Summary:BACKGROUND: Anopheles sinensis is the major malaria vector in China and Southeast Asia. Vector control is one of the most effective measures to prevent malaria transmission. However, there is little transcriptome information available for the malaria vector. To better understand the biological basis of malaria transmission and to develop novel and effective means of vector control, there is a need to build a transcriptome dataset for functional genomics analysis by large-scale RNA sequencing (RNA-seq). METHODS: To provide a more comprehensive and complete transcriptome of An. sinensis, eggs, larvae, pupae, male adults and female adults RNA were pooled together for cDNA preparation, sequenced using the Illumina paired-end sequencing technology and assembled into unigenes. These unigenes were then analyzed in their genome mapping, functional annotation, homology, codon usage bias and simple sequence repeats (SSRs). RESULTS: Approximately 51.6 million clean reads were obtained, trimmed, and assembled into 38,504 unigenes with an average length of 571 bp, an N50 of 711 bp, and an average GC content 51.26%. Among them, 98.4% of unigenes could be mapped onto the reference genome, and 69% of unigenes could be annotated with known biological functions. Homology analysis identified certain numbers of An. sinensis unigenes that showed homology or being putative 1:1 orthologues with genomes of other Dipteran species. Codon usage bias was analyzed and 1,904 SSRs were detected, which will provide effective molecular markers for the population genetics of this species. CONCLUSIONS: Our data and analysis provide the most comprehensive transcriptomic resource and characteristics currently available for An. sinensis, and will facilitate genetic, genomic studies, and further vector control of An. sinensis.
ISSN:1756-3305
1756-3305