Transcription factor TFIIF is not required for initiation by RNA polymerase II, but it is essential to stabilize transcription factor TFIIB in early elongation complexes

Transcription factors TFIIB and TFIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at and downstream of initiation are not clear. We now show that TFIIF phosphorylated by casein kinase 2 remains competent to support PIC assembly but is not stably reta...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2011-09, Vol.108 (38), p.15786-15791
Main Authors: Čabart, Pavel, Újvári, Andrea, Pal, Mahadeb, Luse, Donal S
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biological Sciences
casein
Casein kinase II
Casein Kinase II - genetics
Casein Kinase II - metabolism
DNA
DNA-directed RNA polymerase
Elongation
General transcription factors
Genes
HeLa Cells
Humans
Immunoblotting
Kinases
Phosphorylation
Promoter Regions, Genetic - genetics
Promoters
RNA
RNA polymerase
RNA Polymerase II - metabolism
Templates, Genetic
Transcription Factor TFIIB - genetics
Transcription Factor TFIIB - metabolism
Transcription factors
Transcription Factors, TFII - genetics
Transcription Factors, TFII - metabolism
Transcription initiation factor TFIIB
Transcription Initiation Site
Transcription, Genetic
Trimers
Yeasts
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Summary:Transcription factors TFIIB and TFIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at and downstream of initiation are not clear. We now show that TFIIF phosphorylated by casein kinase 2 remains competent to support PIC assembly but is not stably retained in the PIC. PICs completely lacking TFIIF are not defective in initiation or subsequent promoter clearance, demonstrating that TFIIF is not required for initiation or clearance. Lack of TFIIF in the PIC reduces transcription levels at some promoters, coincident with reduced retention of TFIIB. TFIIB is normally associated with the early elongation complex and is only destabilized at +12 to +13. However, if TFIIF is not retained in the PIC, TFIIB can be lost immediately after initiation. TFIIF therefore has an important role in stabilizing TFIIB within the PIC and after transcription initiates.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1104591108