Molecular Quantification of Respiratory Syncytial Virus in Respiratory Samples: Reliable Detection during the Initial Phase of Infection

Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variati...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 2010-10, Vol.48 (10), p.3569-3574
Main Authors: van de Pol, Alma C, Wolfs, Tom F.W, van Loon, Anton M, Tacke, Carline E.A, Viveen, Marco C, Jansen, Nicolaas J.G, Kimpen, Jan L.L, Rossen, John W.A, Coenjaerts, Frank E.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Humans
Infant
Microbiology
Mucus - virology
Nasopharynx - virology
Respiratory syncytial virus
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Virus, Human - isolation & purification
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Viral - genetics
Trachea - virology
Viral Load
Virology
Virology - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load within one sample (step 1), (ii) variation in the load in samples from patients who were sampled twice on the same day (step 2), and (iii) variation in the load between simultaneously taken nasopharyngeal aspirate (NPA) samples and tracheal aspirate (TA) samples (step 3). Thirty-two infants with RSV infection at the pediatric intensive care unit (PICU) were included. NPA and TA samples were taken three times a week during ventilation and were not diluted. Intrasample variation (step 1) was shown to be minimal (
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00097-10