Multilocus Sequence Typing of Staphylococcus aureus Isolated from High-Somatic-Cell-Count Cows and the Environment of an Organic Dairy Farm in the United Kingdom

An outbreak of Staphylococcus aureus intramammary infections on an organic dairy farm was monitored for 10 months. Environmental and milk samples were collected from a total of 26 cows and a group of 21 purchased heifers about to be introduced into the milking herd. There was variation in the rate o...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-09, Vol.43 (9), p.4731-4736
Main Authors: Smith, E. M, Green, L. E, Medley, G. F, Bird, H. E, Dowson, C. G
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
Cell Count
Clinical Veterinary Microbiology
Dairying
Environment
Female
Fundamental and applied biological sciences. Psychology
Infectious diseases
Mammary Glands, Animal - microbiology
Mastitis, Bovine - epidemiology
Mastitis, Bovine - microbiology
Medical sciences
Microbiology
Milk - microbiology
Miscellaneous
Molecular Sequence Data
Sequence Analysis, DNA
Staphylococcal Infections - veterinary
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
United Kingdom - epidemiology
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Summary:An outbreak of Staphylococcus aureus intramammary infections on an organic dairy farm was monitored for 10 months. Environmental and milk samples were collected from a total of 26 cows and a group of 21 purchased heifers about to be introduced into the milking herd. There was variation in the rate of isolation of S. aureus (9.5 to 43.8%) from individual mammary quarters, although no S. aureus isolates were detected in the milk samples collected from the heifers. One hundred ninety-one S. aureus isolates were detected from cow milk samples (n = 182), milking machine clusters (n = 4), farm personnel (n = 4), and the environment (n = 1). Multilocus sequence typing (MLST) had a typeability of 100% when it was applied to the 191 isolates. Among the 191 isolates there was limited strain diversity, with seven sequence types (STs) dominated by two strains with closely related STs that differed at a single locus. Within individual mammary quarters there were naturally occurring dual infections, although this was identified in only 0.4% of milk samples. Different strains were associated with variable persistence within quarters. MLST is clearly a very suitable tool for the differentiation and analysis of S. aureus populations detected on dairy cattle farms.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.9.4731-4736.2005