Properties of three baculoviruses expressing genes that encode insect-selective toxins: micro-Aga-IV, As II, and Sh I. [Erratum: Mar 1997, v. 8 (3), p. 237.]

We have chemically synthesized and expressed three chimeric genes, mag4, sat2, and ssh1, that encode secretable polypeptides with neurotoxic activity in insects. mag4 encodes the micro-Aga-IV toxin from the spider Agelenopsis aperta and has a mellitin signal sequence from the honey bee Apis mellifer...

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Bibliographic Details
Published in:Biological control 1996-10, Vol.7 (2)
Main Authors: Prikhod'ko, G.G. (University of Georgia, Athens.), Robson, M, Warmke, J.W, Cohen, C.J, Smith, M.M, Wang, P, Warren, V, Kaczorowski, G, Ploeg, L.H.T. van der, Miller, L.K
Format: Article
Language:English
Subjects:
AGELENOPSIS APERTA
ANEMONIA SULCATA
APIS MELLIFERA
AUTOGRAPHA CALIFORNICA
BACULOVIRIDAE
BIOLOGICAL CONTROL
CHIMERIC GENES
CNIDARIA
CONTROL BIOLOGICO
EFFICACY
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
GENE TRANSFER
GENES
GENETIC ENGINEERING
GENETIC TRANSFORMATION
GENETIC VECTORS
GENIE GENETIQUE
INGENIERIA GENETICA
INSECTICIDAL PROPERTIES
INSECTICIDAS
INSECTICIDE
INSECTICIDES
INVERTEBRATE VIRUSES
KILL TIME
LUTTE BIOLOGIQUE
MICROBIAL PESTICIDES
MORTALIDAD
MORTALITE
MORTALITY
NERVOUS SYSTEM
NEUROTOXINS
NUCLEAR POLYHEDROSIS VIRUS
PESTICIDE MICROBIEN
PLAGUICIDAS MICROBIANOS
RECOMBINANT BACULOVIRUS
SARCOPHAGA PEREGRINA
SISTEMA NERVIOSO
SPODOPTERA FRUGIPERDA
STICHODACTYLA HELIATHUS
SYNTHETIC GENES
SYSTEME NERVEUX
TOXIN GENES
TOXINAS
TOXINE
TOXINS
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
TRICHOPLUSIA NI
VIRUS DE INVERTEBRADOS
VIRUS DES INVERTEBRES
VIRUS POLIEDROSIS NUCLEAR
VIRUS POLYEDROSE NUCLEAIRE
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Summary:We have chemically synthesized and expressed three chimeric genes, mag4, sat2, and ssh1, that encode secretable polypeptides with neurotoxic activity in insects. mag4 encodes the micro-Aga-IV toxin from the spider Agelenopsis aperta and has a mellitin signal sequence from the honey bee Apis mellifera. The other two genes, sat2 and ssh1, encode As II and Sh I toxins from the sea anemones Anemonia sulcata and Stichodactyla helianthus, respectively, and have a sarcotoxin signal sequence from the flesh fly Sarcophaga peregrina. The synthetic genes were inserted into a nonessential site in the genome of the Autographa californica nuclear polyhedrosis virus under the control of an enhanced polyhedrin promoter. In all cases, insect cells infected with recombinant viruses produced toxins, and larvae infected with the recombinant viruses died more quickly than larvae infected with wild-type virus. The relative efficacy of the mag4-expressing recombinant was most-dependent. In Trichoplusia ni, sat2 expression was most effective, providing a 38.4% a reduction in time to death compared to the wild-type virus, while in Spodoptera frugiperda, the mag4-expressing recombinant was most effective, exhibiting a 37% reduction in time to death
ISSN:1049-9644
1090-2112