Presence of free D-amino acids in microalgae

Several species of microalgae (phytoplankton), 4 species of freshwater algae and 4 species of marine diatoms, were cultured germ-free in the laboratory. The presence of free D-amino acids was verified in these species by a reversed-phase HPLC analysis. D-Aspartate was detected in all the microalgae...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-02, Vol.67 (2), p.388-392
Main Authors: Yokoyama, T. (Kitasato Univ., Sanriku, Iwate (Japan). School of Fisheries), Kanno, N, Ogata, T, Kotaki, Y, Sato, M, Nagahisa, E
Format: Article
Language:English
Subjects:
ALANINE
Algae
Amino Acids - analysis
Amino Acids - chemistry
ASPARTIC ACID
Asterionella
BACILLARIOPHYCEAE
Biological and medical sciences
CHLORELLA
Chromatography, High Pressure Liquid - methods
D-alanine
D-Amino acids
D-aspartate
diatom
Eukaryota - chemistry
FREE AMINO ACIDS
Fresh Water
Fundamental and applied biological sciences. Psychology
HPLC
microalga
PHYTOPLANKTON
Phytoplankton - chemistry
SCENEDESMUS
Seawater
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Summary:Several species of microalgae (phytoplankton), 4 species of freshwater algae and 4 species of marine diatoms, were cultured germ-free in the laboratory. The presence of free D-amino acids was verified in these species by a reversed-phase HPLC analysis. D-Aspartate was detected in all the microalgae examined, but D-alanine was only present in the marine diatoms. The D-amino acid content in Asterionella sp. of the marine diatoms increased from the exponential phase to the stationary phase and then decreased to the phase of decline.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.388