Synchronization of ovulation and subsequent fertility in buffaloes following PGF2á-PGF2á protocol, with or without GnRH

A PGF2á-based protocol, with or without GnRH, was used to synchronize ovulation and assess subsequent fertility in cycling buffaloes. In protocol-A (n=6) and protocol-B (n=6), buffaloes were administered, at unknown stage of estrous cycle, 2 PGF2á injections (1 mg cloprostenol each) 12 days apart, w...

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Bibliographic Details
Published in:Indian journal of animal sciences 2009-09, Vol.79 (9)
Main Authors: Dadarwal, D, Ghuman, S P S, Honparkhe, M, Singh, Jagir
Format: Article
Language:English
Subjects:
BUFALO DE AGUA
BUFFLE D'EAU
CORPS JAUNE
CORPUS LUTEUM
CUERPO LUTEO
CULTIVO DE OVULOS
CULTURE D'OVULE
http://www.fao.org/aos/agrovoc#c_1905
http://www.fao.org/aos/agrovoc#c_24575
http://www.fao.org/aos/agrovoc#c_6218
http://www.fao.org/aos/agrovoc#c_6236
http://www.fao.org/aos/agrovoc#c_8313
OVULE CULTURE
PROGESTERONA
PROGESTERONE
PROSTAGLANDINAS
PROSTAGLANDINE
PROSTAGLANDINS
WATER BUFFALOES
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Summary:A PGF2á-based protocol, with or without GnRH, was used to synchronize ovulation and assess subsequent fertility in cycling buffaloes. In protocol-A (n=6) and protocol-B (n=6), buffaloes were administered, at unknown stage of estrous cycle, 2 PGF2á injections (1 mg cloprostenol each) 12 days apart, whilst buffaloes of protocol-B received an additional GnRH injection (0.02 mg buserelin) at 48 h after second-PGF2á. Buffaloes were artificially inseminated (AI) twice at 48 and 72 h after second-PGF2á. The luteal profile of day 0 reached basal values in all the buffaloes at 72 h subsequent to first-PGF2á on day 0. The ovulatory follicles observed on day 0, ovulated between 48_120 h subsequent to first-PGF2á in 92 per cent buffaloes. In response to second-PGF2á on day 12, all the buffaloes exhibited luteal regression and displayed onset of estrus around 24 h after second-PGF2á. Ovulation in response to second-PGF2á induced luteolysis was observed in all the buffaloes between 72_96 h after second-PGF2á. Conception of buffaloes was similar across treatments at 42nd day (50%). The administration of GnRH in protocol-B had no effect on time of ovulation or conception. In summary, 2 injections of PGF2á administered 12 day apart in cycling buffaloes were highly efficacious for synchronizing ovulation and thus, permitting fixed-time AI at 72 h after second-PGF2á which results in good enough fertility.
ISSN:0367-8318