Expression of TRPA1 Correlating to the Recovery of Colonic Transit following PN denervation in Rats

Abstract Background It has been reported that colorectal motility dysfunction due to pelvic nerve (PN) damage is restored over time. However, the adaptive mechanism is unknown. Previous studies implied that TRPA1 mediated sensory nerve signal input play a crucial role in gut motility control. The cu...

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Bibliographic Details
Published in:The Journal of surgical research 2016
Main Authors: Tong, Weidong, Tian, Yue, Yang, Hanyong, Wang, Li, Zhao, Song, Shi, Huiwen, Dai, Feixiang, Ye, jingwang
Format: Article
Language:English
Subjects:
Surgery
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Summary:Abstract Background It has been reported that colorectal motility dysfunction due to pelvic nerve (PN) damage is restored over time. However, the adaptive mechanism is unknown. Previous studies implied that TRPA1 mediated sensory nerve signal input play a crucial role in gut motility control. The current study aimed to observe the colorectal motility restoration in rats following PN transection, and to explore the change of TRPA1 protein expression in this adaptive process. Methods 78 adult rats were divided into two groups randomly: sham and PN cut. Colonic transit function was determined with radioisotope method by calculating the geometric center (GC) of the distribution of51 Cr at postoperative day (POD) 1, 3 and 7. Expression of TRPA1 in the proximal and distal colon mucosa was detected with Western Blotting at POD1, 3 and 7. Results At POD 1, the colonic transit in PN cut group was significantly delayed (GC = 4.91±0.41, p < 0.05), when compared with the sham group (GC=5.76±0.85). A significant trend toward recovery was noted in the PN cut group at POD 3 (GC=5.58±0.36) and POD 7 (GC=6.44±0.78). Western blot demonstrated attenuated expression of TRPA1 in the distal colon mucosa after PN denervation at POD1 (0.39±0.12) compared with that of the shams. A significant trend of increasing expression of TRPA1 was demonstrated in the PN cut group at POD 3 (0.78±0.10) and at POD7 (1.06±0.13). Conclusions Delayed colonic motility due to PN denervation gradually restored overtime, which may relate to the corresponding expression of TRPA1 in the distal colonic mucosa of rats.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2016.09.057