Evaluation of Type-Specific Real-Time PCR Assays Using the LightCycler and J.B.A.I.D.S. for Detection of Adenoviruses in Species HAdV-C

Sporadically, HAdVs from species HAdV-C are detected in acute respiratory disease outbreaks. To rapidly type these viruses we designed real-time PCR assays that detect and discriminate between adenovirus types HAdV-C1, -C2, -C5, and -C6. Sixteen clinical isolates from the California Department of Pu...

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Main Authors:	Jones, Morris S, Hudson, Nolan R, Gibbins, Carl, Fischer, Stephen L
Format:	Report
Language:	eng
Subjects:	ACUTE RESPIRATORY DISEASE VIRUS ADENOVIRUSES CLINICAL MEDICINE DIAGNOSTIC EQUIPMENT DISCRIMINATE ANALYSIS HEALTH Medicine and Medical Research REAL TIME RESPIRATORY DISEASES RESPONSE(BIOLOGY) VIRUSES
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Summary:	Sporadically, HAdVs from species HAdV-C are detected in acute respiratory disease outbreaks. To rapidly type these viruses we designed real-time PCR assays that detect and discriminate between adenovirus types HAdV-C1, -C2, -C5, and -C6. Sixteen clinical isolates from the California Department of Public Health were used to validate the new assays. Type-specific TaqMan real-time PCR assays were designed and used independently to successfully identify 16 representative specimens. The lower limit of detection for our LightCycler singleplex real-time PCR assays were calculated to be 100, 100, 100, and 50 genomic copies per reaction for HAdV-C1, HAdV-C2, HAdV-C5 and HAdV-C6, respectively. The results for the singleplex J.B.A.I.D.S. assays were similar. Our assays did not cross-react with other adenoviruses outside of species HAdV-C, respiratory syncytial virus, influenza, or respiratory disease causing bacteria. These assays have the potential to be useful as diagnostic tools for species HAdV-C infection. Pub. in PLoS one , v6 n10, p1-6, Oct 2011.