Inhibitory Activity of Interferons and Interleukin 1 on the Development of Plasmodium falciparum in Human Hepatocyte Cultures

We have studied the effect of natural and recombinant human interferons (HulFN-alpha, beta, and gamma, and interleukin 1 (IL-1) on development of sporozoites of Plasmodium falciparum in cultures of functional hepatocytes. HulFN-gamma inhibits hepatic schizogony of P. falciparum at very low concentra...

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Main Authors: Mellouk, Sylvie, Maheshwari, Radha K, Rhodes-Feuillette, Alice, Beaudoin, Richard L, Berbiguier, Nathalie, Matile, Hughes, Miltgen, Francois, Landau, Irene, Pied, Sylvlvianne, Chigot, Jean P, Friedman, Rovert M, Mazier, Dominique
Format: Report
Language:English
Subjects:
ANTIMICROBIAL AGENTS
CELLS(BIOLOGY)
HUMANS
INHIBITION
INOCULATION
INTERFERON
INTERLEUKIN 1
LIVER
Medicine and Medical Research
PE61102A
Pharmacology
PLASMODIUM FALCIPARUM
REPRINTS
SPOROZOITES
WU427
WUDA301616
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Summary:We have studied the effect of natural and recombinant human interferons (HulFN-alpha, beta, and gamma, and interleukin 1 (IL-1) on development of sporozoites of Plasmodium falciparum in cultures of functional hepatocytes. HulFN-gamma inhibits hepatic schizogony of P. falciparum at very low concentrations the target being the hepatocyte. Application after sporozoite inoculation is effective, suggesting an intracellular mechanism. There is also an 84% inhibition after application from 4 to 6 days following inoculation so that by day 6, there was a disappearance of a significant number of schizonts previously present at day 4, indicating more than a parasitostatic effect, and probably a post assembly action. HulFN-alpha and beta were effective, but only at 1000-fold higher concentrations than HulFN-gamma. IL-1 also inhibited hepatic development of P. falciparum sporozoites; however, IL-1 treatment was effective only when applied before sporozoite inoculation. Reprints. Pub. in Jnl. of Immunology, v139 n12 p4192-4195, 15 Dec 1987.