Heparin and Progesterone Exert Synergistic Effects to Improve the In-Vitro Fertilization Rate of Bovine Sperm Bound to Oviduct Cell Aggregates from the Isthmus

After the copulation process, some sperm start the long journey with an ultimate goal of fertilizing the oocyte. Inside the oviduct, sperm are stored, waiting for the ovulated oocyte where they bind to the apical surface of the oviduct cells, which in turn hold sperm to form a sperm nest. The essent...

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Bibliographic Details
Published in:Veterinary sciences 2022-07, Vol.9 (7), p.372
Main Authors: El-Sokary, Mohamed M. M., Shehata, Seham F., Mahmoud, Karima G. M.
Format: Article
Language:English
Subjects:
Aggregates
bovine
Capacitation
cell aggregates
Cell surface
Copulation
Epithelial cells
Experiments
Fertilization
Heparin
Humidity
Infertility
Life span
Motility
Oocytes
Oviduct
Progesterone
Sperm
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Summary:After the copulation process, some sperm start the long journey with an ultimate goal of fertilizing the oocyte. Inside the oviduct, sperm are stored, waiting for the ovulated oocyte where they bind to the apical surface of the oviduct cells, which in turn hold sperm to form a sperm nest. The essential functions of the sperm reservoir include attaching spermatozoa to oviduct epithelial cells, selecting intact, good-quality sperm with an end result of extending sperm life expectancy. The current study aimed to evaluate the fertilization ability of sperm that bind to cell aggregates from different parts of the oviduct (infundibulum-ampulla-isthmus), and to assess the effect of heparin and or progesterone (P4) on the in-vitro fertilization ability of sperm co-incubated with cell aggregates from the isthmus. In-vitro fertilization was identified as a cleaved oocyte to two cells or more. The sperm bound to cell aggregates from the isthmus improved the rate of in-vitro fertilization (48.09%) compared to aggregates from the infundibulum (36.90%) and ampulla (37.61%). Moreover, pre-treatment of mature COCs with heparin (40 μg/mL) and P4 (80 nanomolar) play a coactive role that improves the in-vitro fertilization ability of sperm bound to cell aggregates from isthmus (63.33%), compared to 42.61% in the absence of cells aggregates. In conclusion, binding to cell aggregates from isthmus improves the in-vitro fertilization ability of Bovine sperm. Additionally, heparin, together with P4, exerts a synergistic action that improves the in-vitro fertilizing potential of sperm attached to cell aggregates from the isthmus of the bovine oviduct.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci9070372