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Development of a confinable gene drive system in the human disease vector Aedes aegypti
is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of...
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| Published in: | eLife 2020-01, Vol.9 |
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| creator | Li, Ming Yang, Ting Kandul, Nikolay P Bui, Michelle Gamez, Stephanie Raban, Robyn Bennett, Jared Sánchez C, Héctor M Lanzaro, Gregory C Schmidt, Hanno Lee, Yoosook Marshall, John M Akbari, Omar S |
| description | is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in
. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of
to combat local pathogen transmission. |
| doi_str_mv | 10.7554/elife.51701 |
| format | article |
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. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of
to combat local pathogen transmission.</description><identifier>ISSN: 2050-084X</identifier><identifier>EISSN: 2050-084X</identifier><identifier>DOI: 10.7554/elife.51701</identifier><identifier>PMID: 31960794</identifier><language>eng</language><publisher>England: eLife Science Publications, Ltd</publisher><subject>Aedes - genetics ; Aedes - physiology ; Aedes aegypti ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - physiology ; Cas9 ; CRISPR ; CRISPR-Cas Systems - genetics ; dengue ; Dengue fever ; Disease transmission ; Efficiency ; Epidemiology and Global Health ; Female ; Gene Drive Technology ; Genes ; Genetic aspects ; Genetic control ; Genomes ; Health aspects ; Infection ; Male ; Mathematical models ; Medical research ; Mosquito Vectors - genetics ; Mosquito Vectors - physiology ; Mosquitoes ; Mutation ; Organisms ; Pathogens ; Population ; RNA, Guide, CRISPR-Cas Systems - genetics ; split gene drives ; Tropical diseases ; Vectors (Biology)</subject><ispartof>eLife, 2020-01, Vol.9</ispartof><rights>2020, Li et al.</rights><rights>COPYRIGHT 2020 eLife Science Publications, Ltd.</rights><rights>2020, Li et al. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020, Li et al 2020 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c642t-475b3732405a1c1c7713d980fefb1bd2416db29fe846fac89957f392cc667c3a3</citedby><cites>FETCH-LOGICAL-c642t-475b3732405a1c1c7713d980fefb1bd2416db29fe846fac89957f392cc667c3a3</cites><orcidid>0000-0001-8915-891X ; 0000-0002-7578-4968 ; 0000-0001-7201-4231 ; 0000-0003-0603-7341 ; 0000-0002-5648-6770 ; 0000-0002-6853-9884</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2349165190/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2349165190?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,730,783,787,888,25767,27938,27939,37026,37027,44604,53806,53808,75462</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31960794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ming</creatorcontrib><creatorcontrib>Yang, Ting</creatorcontrib><creatorcontrib>Kandul, Nikolay P</creatorcontrib><creatorcontrib>Bui, Michelle</creatorcontrib><creatorcontrib>Gamez, Stephanie</creatorcontrib><creatorcontrib>Raban, Robyn</creatorcontrib><creatorcontrib>Bennett, Jared</creatorcontrib><creatorcontrib>Sánchez C, Héctor M</creatorcontrib><creatorcontrib>Lanzaro, Gregory C</creatorcontrib><creatorcontrib>Schmidt, Hanno</creatorcontrib><creatorcontrib>Lee, Yoosook</creatorcontrib><creatorcontrib>Marshall, John M</creatorcontrib><creatorcontrib>Akbari, Omar S</creatorcontrib><title>Development of a confinable gene drive system in the human disease vector Aedes aegypti</title><title>eLife</title><addtitle>Elife</addtitle><description>is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in
. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of
to combat local pathogen transmission.</description><subject>Aedes - genetics</subject><subject>Aedes - physiology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Animals, Genetically Modified - genetics</subject><subject>Animals, Genetically Modified - physiology</subject><subject>Cas9</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>dengue</subject><subject>Dengue fever</subject><subject>Disease transmission</subject><subject>Efficiency</subject><subject>Epidemiology and Global Health</subject><subject>Female</subject><subject>Gene Drive Technology</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic control</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Infection</subject><subject>Male</subject><subject>Mathematical models</subject><subject>Medical research</subject><subject>Mosquito Vectors - genetics</subject><subject>Mosquito Vectors - physiology</subject><subject>Mosquitoes</subject><subject>Mutation</subject><subject>Organisms</subject><subject>Pathogens</subject><subject>Population</subject><subject>RNA, Guide, CRISPR-Cas Systems - genetics</subject><subject>split gene drives</subject><subject>Tropical diseases</subject><subject>Vectors (Biology)</subject><issn>2050-084X</issn><issn>2050-084X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkt2LEzEUxQdR3GXdJ98l4IuytOZrksmLUNZVCwXBD_QtZJKbacrMpE5miv3vTbfruhWTh4Sb3z0hJ6conhM8l2XJ30AbPMxLIjF5VJxTXOIZrviPxw_2Z8VlShuch-RVRdTT4owRJbBU_Lz4_g520MZtB_2IokcG2dj70Ju6BdRAD8gNYQco7dMIHQo9GteA1lNneuRCApMA7cCOcUALcJCQgWa_HcOz4ok3bYLLu_Wi-Pb-5uv1x9nq04fl9WI1s4LTccZlWTPJKMelIZZYKQlzqsIefE1qRzkRrqbKQ8WFN7ZSqpSeKWqtENIywy6K5VHXRbPR2yF0ZtjraIK-LcSh0WYYg21Be0spdQoqKmuuHNRYmVIKnOWpq6zPWm-PWtup7sDZbMlg2hPR05M-rHUTd1ooyZkgWeDVncAQf06QRt2FZKFtTQ9xSpoyzjCrSkEz-vIfdBOnoc9WHShFREkU_ks1Jj8g9D7me-1BVC8EIVXFGBWZmv-HytNBF_J3gg-5ftLw-qQhMyP8GhszpaSXXz6fsldH1g4xpQH8vR8E60MENaxyBPVtBDP94qGF9-yfwLHfCgDU7A</recordid><startdate>20200121</startdate><enddate>20200121</enddate><creator>Li, Ming</creator><creator>Yang, Ting</creator><creator>Kandul, Nikolay P</creator><creator>Bui, Michelle</creator><creator>Gamez, Stephanie</creator><creator>Raban, Robyn</creator><creator>Bennett, Jared</creator><creator>Sánchez C, Héctor M</creator><creator>Lanzaro, Gregory C</creator><creator>Schmidt, Hanno</creator><creator>Lee, Yoosook</creator><creator>Marshall, John M</creator><creator>Akbari, Omar S</creator><general>eLife Science Publications, Ltd</general><general>eLife Sciences Publications Ltd</general><general>eLife Sciences Publications, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8915-891X</orcidid><orcidid>https://orcid.org/0000-0002-7578-4968</orcidid><orcidid>https://orcid.org/0000-0001-7201-4231</orcidid><orcidid>https://orcid.org/0000-0003-0603-7341</orcidid><orcidid>https://orcid.org/0000-0002-5648-6770</orcidid><orcidid>https://orcid.org/0000-0002-6853-9884</orcidid></search><sort><creationdate>20200121</creationdate><title>Development of a confinable gene drive system in the human disease vector Aedes aegypti</title><author>Li, Ming ; Yang, Ting ; Kandul, Nikolay P ; Bui, Michelle ; Gamez, Stephanie ; Raban, Robyn ; Bennett, Jared ; Sánchez C, Héctor M ; Lanzaro, Gregory C ; Schmidt, Hanno ; Lee, Yoosook ; Marshall, John M ; Akbari, Omar S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c642t-475b3732405a1c1c7713d980fefb1bd2416db29fe846fac89957f392cc667c3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Aedes - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>eLife</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ming</au><au>Yang, Ting</au><au>Kandul, Nikolay P</au><au>Bui, Michelle</au><au>Gamez, Stephanie</au><au>Raban, Robyn</au><au>Bennett, Jared</au><au>Sánchez C, Héctor M</au><au>Lanzaro, Gregory C</au><au>Schmidt, Hanno</au><au>Lee, Yoosook</au><au>Marshall, John M</au><au>Akbari, Omar S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a confinable gene drive system in the human disease vector Aedes aegypti</atitle><jtitle>eLife</jtitle><addtitle>Elife</addtitle><date>2020-01-21</date><risdate>2020</risdate><volume>9</volume><issn>2050-084X</issn><eissn>2050-084X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in
. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of
to combat local pathogen transmission.</abstract><cop>England</cop><pub>eLife Science Publications, Ltd</pub><pmid>31960794</pmid><doi>10.7554/elife.51701</doi><orcidid>https://orcid.org/0000-0001-8915-891X</orcidid><orcidid>https://orcid.org/0000-0002-7578-4968</orcidid><orcidid>https://orcid.org/0000-0001-7201-4231</orcidid><orcidid>https://orcid.org/0000-0003-0603-7341</orcidid><orcidid>https://orcid.org/0000-0002-5648-6770</orcidid><orcidid>https://orcid.org/0000-0002-6853-9884</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Aedes - genetics Aedes - physiology Aedes aegypti Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - physiology Cas9 CRISPR CRISPR-Cas Systems - genetics dengue Dengue fever Disease transmission Efficiency Epidemiology and Global Health Female Gene Drive Technology Genes Genetic aspects Genetic control Genomes Health aspects Infection Male Mathematical models Medical research Mosquito Vectors - genetics Mosquito Vectors - physiology Mosquitoes Mutation Organisms Pathogens Population RNA, Guide, CRISPR-Cas Systems - genetics split gene drives Tropical diseases Vectors (Biology) |
| title | Development of a confinable gene drive system in the human disease vector Aedes aegypti |
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