Development of a confinable gene drive system in the human disease vector Aedes aegypti

is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of...

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Bibliographic Details
Published in:eLife 2020-01, Vol.9
Main Authors: Li, Ming, Yang, Ting, Kandul, Nikolay P, Bui, Michelle, Gamez, Stephanie, Raban, Robyn, Bennett, Jared, Sánchez C, Héctor M, Lanzaro, Gregory C, Schmidt, Hanno, Lee, Yoosook, Marshall, John M, Akbari, Omar S
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes - physiology
Aedes aegypti
Animals
Animals, Genetically Modified - genetics
Animals, Genetically Modified - physiology
Cas9
CRISPR
CRISPR-Cas Systems - genetics
dengue
Dengue fever
Disease transmission
Efficiency
Epidemiology and Global Health
Female
Gene Drive Technology
Genes
Genetic aspects
Genetic control
Genomes
Health aspects
Infection
Male
Mathematical models
Medical research
Mosquito Vectors - genetics
Mosquito Vectors - physiology
Mosquitoes
Mutation
Organisms
Pathogens
Population
RNA, Guide, CRISPR-Cas Systems - genetics
split gene drives
Tropical diseases
Vectors (Biology)
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Summary:is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in . To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of to combat local pathogen transmission.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.51701