Systems-based approach to examine the cytokine responses in primary mouse lung macrophages infected with low pathogenic avian Influenza virus circulating in South East Asia

Influenza A virus (IAV) is a major public health concern, being responsible for the death of approximately half a million people each year. Zoonotic transmissions of the virus from swine and avian origin have occurred in the past, and can potentially lead to the emgergence of new IAV stains in f...

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Published in:	BMC genomics 2017-05, Vol.18 (1), p.420-420, Article 420
Main Authors:	Taye, Biruhalem, Chen, Hui, Myaing, Myint Zu, Tan, Boon Huan, Maurer-Stroh, Sebastian, Sugrue, Richard J
Format:	Article
Language:	eng
Subjects:	Adaptation Animals Asia Avian flu Avian influenza virus Cell Death - drug effects Cell Death - genetics Chemokines Cytokines Cytokines - genetics Drug resistance Gene expression Gene Expression Profiling Genomics H5N2 H5N3 Host-Pathogen Interactions - genetics Host-Pathogen Interactions - immunology Infections Inflammation Inflammatory response Influenza Influenza A Influenza A Virus, H1N1 Subtype - physiology Influenza A Virus, H5N2 Subtype - physiology Interferons - pharmacology Livestock Lung - immunology Lung - virology Lungs Macrophage Activation - drug effects Macrophage Activation - genetics Macrophages Macrophages - drug effects Macrophages - immunology Macrophages - metabolism Mice Molecular Sequence Annotation Pandemics Public health Swine Viral infections Viruses Zoonoses
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Summary:	Influenza A virus (IAV) is a major public health concern, being responsible for the death of approximately half a million people each year. Zoonotic transmissions of the virus from swine and avian origin have occurred in the past, and can potentially lead to the emgergence of new IAV stains in future pandemics. Pulmonary macrophages have been implicated in disease severity in the lower airway, and understanding the host response of macrophages infected with avian influenza viruses should provide new therapeutic strategies. We used a systems-based approach to investigate the transcriptome response of primary murine lung macrophages (PMФ) infected with the mouse-adapted H1N1/WSN virus and low pathogenic avian influenza (LPAI) viruses H5N2 and H5N3. The results showed that the LPAI viruses H5N2 and H5N3 can infect PMФ with similar efficiency to the H1N1/WSN virus. While all viruses induced antiviral responses, the H5N3 virus infection resulted in higher expression levels of cytokines and chemokines associated with inflammatory responses. The LPAI H5N2 and H5N3 viruses are able to infect murine lung macrophages. However, the H5N3 virus was associated with increased expression of pro-inflammatory mediators. Although the H5N3 virus it is capable of inducing high levels of cytokines that are associated with inflammation, this property is distinct from its inability to efficiently replicate in a mammalian host.
ISSN:	1471-2164 1471-2164